放置时间及保存温度对幼儿末梢全血细胞计数的影响

The influence of storage time and preserving temperature on infant children′s peripheral blood cell count

目的探讨放置时间、保存温度对2～6岁幼儿末梢全血细胞计数结果的影响,以指导体检工作流程的合理安排。方法取40名幼儿末梢全血用乙二胺四乙酸三钾(EDTA-K3)抗凝,分组标记后保存于不同温度中。应用ABX-Micros 60全自动血液分析仪,将分装标本分别在0.5、4、8和10 h测定。结果保存于室温和4℃冰箱冷藏环境下,随着放置时间的延长,白细胞(WBC)、红细胞(RBC)、平均血小板体积(MPV)、血小板分布宽度(PDW)、淋巴细胞百分率(LY)均有逐渐上升的趋势;血小板(PLT)计数、中性粒细胞百分率(GRA)均有下降的趋势。标本于室温8 h及4℃冷藏10 h以后检测结果与对照组比较,大多数指标差异均有统计学意义(P<0.05)。10 h时室温与4℃冷藏放置2组数据比较,大多数指标差异有统计学意义(P<0.05)。结论标本放置时间及保存温度对幼儿末梢全血细胞计数结果中的一些参数有明显影响,在4h之内检测,室温与4℃冷藏2种环境对检测结果无明显影响;末梢血标本放置在4℃冷藏条件下10h内完成检测还是可行的。

Objective To investigate the influence of storage time and preserving temperature on 2-6 years old infant children′s peripheral blood cell count,and improve the process of health examination.Methods 40 cases of infant children′s peripheral blood specimens were anticoagulated by ethylenediamine tetraacetic acid-K3（EDTA-K3）.The blood specimens were classified and stored in different temperatures after being labeled.The blood specimens were determined by ABX-Micros 60 at 0.5,4,8 and 10 h respectively.Results The levels of white blood cell（WBC）,red blood cell（RBC）,mean platelet volume（MPV）,platelet distribution width（PDW）,lymphocyte%（LY） were increased,and platelet（PLT） and nentrophilicgranulocyte %（GRA） were decreased with prolonging storage time in room temperature and refrigerator 4 ℃.After 8 h in room temperature and 10 h in 4 ℃,most of the results had significant differences compared with the control group（P0.05）.There were significant differences between the results of 10 h in room temperature and in 4 ℃ with most of the results（P0.05）.Conclusions Storage time and preserving temperature have significant influence on the part results of infant children′s peripheral blood cell count.The results are not different significantly between within 4 h in room temperature or 4 ℃ in refrigerator.The results would be acceptable,if the specimens are stored in refrigerator 4 ℃ within 10 h.