维生素D受体在斑秃皮损处毛囊的异常表达

Abnormal expression of VDR in the hair follicle of alopecia areata

目的观察维生素D受体(VDR)在斑秃患者及正常人毛囊中的表达情况。方法分别通过荧光定量PCR及免疫组化法检测24例斑片型斑秃患者及12例正常人头皮生长期毛囊中维生素D受体的mRNA及蛋白表达水平,并比较斑秃患者不同脱发面积、发病年龄、病程与维生素D受体表达水平高低之间关系。结果斑秃患者生长期毛囊VDR的mRNA及蛋白表达水平显著低于于正常人,但不同发病年龄、脱发面积、病程斑秃患者VDR表达水平差异无显著性。结论维生素D受体在斑秃患者毛囊的表达低于正常人,可能在斑秃发病中起作用。

Objective To detect the expression of Vitamin D receptor（VDR） in the hair follicles of alopecia areata and healthy subjects. Methods Expression of VDR at both protein and mRNA levels in hair follicles of 12 controls and 24 alopecia areata（AA） patients were examined by immunohistochemistry and fluorescence quantitative PCR,respectively.VDR expression among AA patients with different onset age,disease course and hair loss area was analyzed. Results Compared with normal controls,the expression of VDR in hair follicle at protein and mRNA levels in AA were significantly reduced.However,there was no significant difference in VDR expression among AA patients with different onset age,disease course and hair loss area. Conclusion Low VDR protein and mRNA expression in the hair follicles of AA patients may play an important role in pathogenesis of AA.