镉中毒肾损害大鼠尿中金属硫蛋白的变化

The Changes of Urinary Metallotionein in Cd-induced Renal Damage Rats

用Sephadex G-75凝胶层析技术对亚急性镉中毒肾损害大鼠肝、肾、血、尿中金属硫蛋白(MT)进行了分离测定。结果表明,大鼠接受镉后,其肝、肾、血、尿中MT增多;MT是体内镉的主要存在形式;尿中MT增多是镉中毒肾损害最早出现的变化之一,是肾小管功能障碍的灵敏指标,对反映镉性肾损害有其特异性。

Male Wistar rats were treated with CdCl2 ( 0.592mgCa++/kg, ip., 5 times a week up to 9 weeks). Metallothionein(MT ) in the urine, blood and supernatants of liver and renal cortex ho mogenates were isolated by Sephadex G-75 column chromatography and measured by UV-AAS. MT levels in them were increased after cadmium exposure. Most of the cadmium were bound to MT. These suggest that MT may play a major role in transfer, accnmulation and excretionof cadmium in the body. Increase of MT in urine was not only the earliest change as a low molecular weight protein, but also the specific index of Cd-induced renal damage as a Cd-binding protein.