摘要
向大鼠肝微粒体膜悬液中加入CCl_4(0.05～1μl/ml),可致膜脂过氧化和膜脂流动性降低。二巯基苏糖醇(DTT)和普鲁卡因能拮抗上述效应。CCl_4与微粒体膜作用后,ANS荧光强度降低并出现红移。结果提示,CCl_4诱发的膜脂过氧化可致微粒体膜的物理状态发生改变。
The addition of CCl4 ( 0.05-μ1/ml ) to rat liver microsome resulted in the membrane peroxi dation and decrease in lipid fluidity. DithiothreitoKDTT ) and procaine were found to be antago nistic. The fluorescence intensity of ANS was decreased and red-shifted after CCl4 treatment of the membranes. The results suggest the changes in the physical state of microsomes subsequent to CCl4 induced peroxidation of the membrane lipids.
出处
《卫生毒理学杂志》
CSCD
1990年第3期157-159,165,共4页
Journal of Health Toxicology
关键词
四氯化碳
肝
微粒体
过氧化脂质
Carbon tetrachloride
Liver microsome
Lipid peroxidation
Fluidity of membrane lipid
ANS fluorescence intensity
