中华蟾蜍中脑组织学研究

Histological Structure on Midbrain of Bufo gargarizans

采用HE染色和Holmes银染法对蟾蜍中脑的显微结构进行了研究。中脑背侧,视叶可分为顶盖和被盖,顶盖从外侧到内侧依次分为:带状层、外灰质层、浅白质层、中灰质层、中白质层、深灰质层、深白质层和中央灰质。被盖前端分层与顶盖相同,后端分层不明显。中脑腹侧包括被盖和大脑脚,HE染色和Holmes银染法显示,大脑脚从外向内颜色由浅变深,存在大量纵向神经纤维束,两脚底分界处有横向交错的神经纤维。被盖外侧细胞不分层,聚集形成核团。被盖内侧,细胞和纤维以中脑水管为中心,呈同心圆环分8层。通过比较蟾蜍中脑背腹差异程度,了解背腹功能不同。同时对中华蟾蜍中脑同其他脊椎动物的进行了比较。

HE staining and Holmes silver staining methods were used to reveal the histological structure of the midbrain in Bufo gargarizans.In dorsal midbrain,the optic lobe can be divided into tectum and tegmentum.From lateral to medical,tectum includes eight layers： stratum zonale,outer gray layer,superficial white layer,middle gray layer,middle white layer,deep gray layer,deep white layer and central gray substance.The anterior of tegmentum organization is the same as tectum,but the layers in the posterior of tegmentum are not obvious.Ventral midbrain includes the tegmentum and the cerebral peduncle.The color of cerebral peduncle becomes darker from lateral to medical by HE staining and Holmes silver staining,there are a large number of longitudinal nerve fibers in cerebral peduncle.It＇s the transverse nerve fibers in the boundary department of two crus cerebris.The cells in lateral tegmentum are not stratified and gathered into nucleus.In medialis tegmetum,cells and fibers are sub-eight layers concentric rings as the center of the midbrain aqueduct.By comparing with the difference of toads＇ midbrain dorsal and ventral,we can understand different functions of the dorsal and ventral.The characters of midbrain in Bufo gargarizans were compared with other vertebrate animals.