不同强度有氧运动对糖尿病大鼠肝脏炎症状态的影响

Effect of different intensity exercise to liver inflammatory state of rats with diabetic mellitus

为探讨不同强度有氧运动对糖尿病大鼠肝脏炎症状态的影响,为糖尿病的治疗提供新思路。通过高脂饲料喂养并注射链脲佐菌素的方法,建立SPF级雄性SD大鼠糖尿病模型,将造模成功后的43只大鼠随机分为:糖尿病安静对照组(DMC)10只,糖尿病运动1组(DME1)、糖尿病运动2组(DME2)、糖尿病运动3组(DME3)各11只。运动组大鼠分别在跑台上进行10、15和20 m/min的有氧运动,1 h/d,5 d/周,持续运动6周。实验第6周末检测糖代谢相关指标、血清与肝脏游离脂肪酸,以及反映炎症状态的肿瘤坏死因子(TNF-α),并对各指标进行Person相关性分析。结果发现:各运动组FBG均出现极显著性下降(P<0.01);DME1、DME2组GSP下降具有统计学意义(P<0.05、P<0.01);DME3组FINS的上升具有显著性意义(P<0.05),且DME1、DME2与DME3组间差异也存在显著性(P<0.05);DME1、DME2组IRI出现显著性下降(P<0.05),而DME3组却显示上升(P>0.05),并且显著性或极显著性高于DME1、DME2组(P<0.05、P<0.01);各运动组肝脏TNF-α均出现不同程度的下降,但只有DME2组具有统计学意义(P<0.01)。经Person相关性分析,肝脏TNF-α与FPG、IRI正相关。结果说明:6周的有氧运动能够降低糖尿病大鼠肝脏TNF-α含量,改善肝脏炎症状态,同时改善糖尿病大鼠的糖代谢紊乱和胰岛素抵抗,缓解糖尿病的发展。其中,中等强度有氧运动干预效果较好。

This study is to discuss the anti-inflammatory effect of different intensity exercise, providing new ideas for the treatment of diabetes. Rat model of diabetes were established by high-fat diet with low-dose streptozotocin intraperitoneally injected, and randomly divided into diabetes control group （DMC）, and 3 exercising groups （DME1, DME2, DME3）. After adapting to treadmill exercise, exercise groups underwent treadmill training at the speed of 10 m/min （DME1）, 15 m/min（DME2） and 25 m/min（DME3）, 1 h/d, 5 d/week, continuous training for 6 weeks. After 6 weeks, the histopathologic changes of livers were evaluated.the serum ALT, AST, the liver NF-κB content were tested. Results： The exercise group FBG were significant decrease （P〈0.01）; DME1, DME2 group of GSP decreased significantly （P〈0.05, P〈0.01）; FINS was increase significantly in DME3 Group （P〈0.05）, and DME1, DME2 and DME3 group there was significant difference （P〈0.05）; DME1, DME2 group IRI were decreased （P〈0.05）, while the DME3 groups was increased significantly （P〉0.05）, and significantly than DME1, DME2 group （P〈0.05, P〈0.01）; the exercise group suffered from hepatic TNF-α decreased to different extent, but only DME2 group was decrease significantly （P〈0.01）. The Person correlation analysis, liver TNF-α and FPG, IRI is related.Conclusion： Medium-intensity exercise may be better to control blood glucose levels of diabetes and decrease the levels of TNF-α in the liver, thereby improving the status of inflammation in the liver of diabetes, and it is better to prevent and treat diabetic osteoporosis, but its mechanism remains to be further studied.