摘要
基于抗坏血酸将Fe3+还原为Fe2+,用邻二氮菲作Fe2+的显色剂,用pH 5.4的六亚甲基四胺-HCl缓冲溶液调节体系酸度,建立了测定抗坏血酸含量的新方法,即显色光度法测定药物中的抗坏血酸。讨论了最佳的反应条件,结果表明抗坏血酸浓度在0～10μg/mL范围内符合比尔定律。此方法可应用于药品、合成样品中抗坏血酸含量的测定。
According to ascorbic acid be sour to restore Fe3＋ as Fe2＋ and use O-Philippines make Fe2＋show a color,in the conditions of the hexamethylene tetramine-HCl buffer solution（pH 5.4）.The new measurement method of Ascorbic acid was built up,that is Color spectrophotometry measurement method of ascorbic acid.The potimum measruement condition was studied.The linear rang determination was 0～10 ？g/mL,the method can be for the measurement in the drugs and Synthetic samples.
出处
《广东化工》
CAS
2011年第5期199-200,219,共3页
Guangdong Chemical Industry
关键词
邻二氮菲
显色光度法
抗坏血酸
O-Philippines
color spectrophotometry
ascorbic acid
