同时分离与测定鸡血中的五种喹诺酮药物的高效液相色谱法研究

The Simultaneous Determination of Five Quinolones in Chicken Plasma Sample Using Reversed-Phase High Performance Liquid Chromatography

建立同时分离和测定五种喹诺酮类药物的高效液相色谱分析方法。本实验采用VP-ODS色谱柱,以甲醇-磷酸二氢钠缓冲溶液为流动相。五种喹诺酮类药物测定线性范围0.05～50.0μg/mL,最低检测限为0.003～0.005μg/mL。本方法简便、快速、灵敏度高,并运用于鸡体内的测定。

High performance liquid chromatography（HPLC） was developed for the simultaneous determination of five quinolones in chicken plasma.The experiments of HPLC were carried out on a VP-ODS column using phosphate buffer solution： methanol.The calibration curves were linear in the concentration range from 0.05～50.0 μg/mL.The detection limit of five QNs was 0.003～0.005 μg/mL.The method was validated for accuracy and precision,and it was successfully applied to plasma samples from chicken that had been orally administered.