微型种植体支抗-骨界面压力侧和非压力侧的组织学研究

A histological study on the bone-implant interface of the mini-screw implant anchorage

目的 研究在不同愈合时间和负载条件下微型种植体支抗-骨界面压力侧和非压力侧的组织学变化.方法 选用纯种Beagle犬8只,在其上颌根间牙槽骨中植入86颗微型种植体支抗,分别进行即刻负载、愈合2周、愈合4周、愈合12周后加载150 g和300 g牵引力,定期注射荧光标志物,加载2个月后处死动物.标记种植体的压力侧和非压力侧,两侧分别进行种植体颈部骨结合率（BIC）、骨充填率（BSA）和矿物沉积率（MAR）的测量以及HE染色组织形态观察,以BIC和BSA作为骨整合能力的观察指标.结果 即刻负载组和愈合2周负载300克组压力侧的骨整合能力、矿物沉积率和骨改建活跃程度均小于非压力侧,其余各组则相反.不同负载力值组间比较无显著性差异.结论 微型种植体支抗-骨界面颈部即刻负载时压力侧的骨整合和骨改建较非压力侧低,随着愈合时间的延长,压力侧相对增强,非压力侧相对降低,而负载力值对界面的影响较小.

Objective To investigate the histological difference at the bone-implant interface between pressure and non-pressure sides of the mini-screw implant anchorage （MIA） under the condition of different loading force and heeling time. Methods Eighty-six MIAs were inserted in the maxillary alveolar bone between the dental roots in 8 beagles. Healing after 0, 2, 4, 12 weeks, 150 g and 300 g forces were loaded perpendicularly to the implants and fluorochrome was injected. Two months later, all dogs were sacrificed. The slices of MIA on the pressure and non-pressure sides were labeled. Bone implant contact ratio（BIC）, bone surface area（BSA） and mineral apposition rate （MAR） were measured and histological observations were carried out. Results BIC, BSA, MAR and bone remodeling on the pressure side were lower than those on the non-pressure side in the immediate loading group and the group healing after 2 weeks with 300 g force, while the reverse result was observed in the rest of the groups. No significant difference was found between the two different force loading groups. Conclusions Bone integration and remodeling on the pressure side of MIA-bone interface was lower than those on the non-pressure side in the immediate loading group. With the healing time, it was relatively increased on the pressure side while decreased on the non-pressure side.