摘要
Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation.Like other herbal medicines,S.ningpoensis collected from different localities may considerably differ in their therapeutic efficacy,and the one grown in Zhejiang Province is recognized as geo-authentic.However,it is difficult to confirm the geo-graphical authenticity by similar morphological characteristics.In the present study,inter-simple sequence repeat(ISSR) markers were conducted to detect S.ningpoensis from different origins.A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones.By cloning and sequencing that specific band,sequence characterized amplified region(SCAR) markers were designed to distinguish geo-authentic S.ningpoensis from others.This is a rapid and easy method that can be used to identify the geographical authenticity of S.ningpoensis.
Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation. Like other herbal medicines, S. ningpoensis collected from different localities may considerably differ in their therapeutic efficacy, and the one grown in Zhejiang Province is recognized as geo-authentic. However, it is difficult to confirm the geo- graphical authenticity by similar morphological characteristics. In the present study, inter-simple sequence repeat （ISSR） markers were conducted to detect S. ningpoensis from different origins. A 1259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones. By cloning and sequencing that specific band, sequence characterized amplified region （SCAR） markers were designed to distinguish geo-authentic S. ningpoensis from others This is a rapid and easy method that can be used to identify the geographical authenticity of S. ningpoensis.
基金
Project supported by the National Basic Research Program (973) of China(No.2007CB411600)
the National Natural Science Foundation of China(No.31070205)
the Key Agricultural Program of Pan’an County of Zhejiang Province,China(No.2005ZB01)
