大鼠急性心肌梗死后心肌组织中Wnt-1和β-catenin的mRNA表达

Wnt-1 and β-catenin mRNA expressions in rats＇ myocardial tissue after acute myocardial infarction

目的探讨Wnt-1，β—catenin在大鼠急性心梗后心肌组织中mRNA的表达及意义。方法成年雄性SD大鼠108只，由阜外医院实验动物中心提供。将大鼠随机（随机数字法）分成2组，对照组（c组）和实验组，每组54只。实验组动物的心脏分为梗死区与非梗死区，分设为Ⅰ组（梗死组）和N组（非梗死组）。建立在体大鼠急性心梗模型，模型制作成功后分别于术后1，4，7，10，14，28d各处死9只动物；对照组只穿线，不结扎血管。采用实时荧光定量PCR（real—time fluorescent quantitative polymerase chain reaction，RT—FQ—PCR）技术检测Wnt-1，β—cateninmRNA在C组、I组和N组心肌组织中的时空表达，同时观察心梗后心肌组织的病理学变化。采用SAS软件的q检验和秩和检验进行统计学分析。结果心肌组织中Wnt-1、β—catenin的mRNA表达水平在c组与N组中差异无统计学意义；Ⅰ组1，4，7，10，14d心肌组织中的表达水平较C组、N组均明显增高（P〈0．01），且动态变化与心梗后心肌的愈合过程相平行。结论Wnt信号通路参与、调控急性心梗后心肌组织的修复和愈合过程。

Objective To investigate the mRNA expressions and changes of both Wnt-1 and β-catenin in rats＇ myocardial tissue after acute myocardial infarction. Method A total of 108 adult rats from Bei- jing Fuwai hospital animal experience center were randomized （random number） into experimental group （n = 54） and control group （ n = 54 ）. The myocardial tissue in experimental group was subgrouped as per tis- sue from infarction region and non-infarction region （group I and group N）. After acute myocardial infarction model of rat was set up, the reverse transcription fluorescent quantitative polymerase chain reaction （ RT-FQ- PCR） was used to detect the spatio-temporal mRNA expressions of both Wnt-1 and β-catenin in rats＇ myocar- dial tissue from infarction zone （ group I ）, tissue from non-infarction zone （ group N ） and control group （group C）. Pathological changes of myocardial tissue after infarction were also observed. Results There were no significant differences in both Wnt-1 and β-eatenin mRNA expressions in myocardial tissue between group C and group N, However, their expressions in group I were significantly higher than those in either group C or group N on the 1 st, 4th, 7th, 10th and 14th days （ P 〈 0.01 ）. The dynamic changes of both expressions varied in paralleled with myocardial healing processes after myocardial infarction. Conclusions Wnt signal pathway might participate and regulate the repair and healing processes of myocardial tissue after a- cute myocardial infarction.