GnRHa和避孕药保护化疗所致卵巢损伤的比较研究

Comparison study of the prevention of chemotherapy-induced ovarian damage by gonadotropin-releasing hormone agonist and oral contraceptive in rat model

目的:探讨促性腺激素释放激素激动剂(GnRHa)和口服避孕药(OC,达英-35)对注射环磷酰胺(CTX)大鼠卵巢功能的保护作用。方法:实验分为6组:空白对照组、CTX组、OC组、GnRHa组、OC保护组、GnRHa保护组,每组15只,分别接受生理盐水、CTX、达英-35、GnRHa、达英-35+CTX、GnRHa+CTX注射或灌胃。阴道涂片观察大鼠动情周期,以放射免疫法检测血清雌二醇(E2)和卵泡刺激素(FSH)浓度,每组在停药当天、15天及30天分别处死5只大鼠,观察卵巢重量、卵巢结构及各级卵泡数目。结果:停药后30天,OC保护组和GnRHa保护组的E2浓度分别为51.33±2.00pg/ml、44.38±5.98pg/ml,FSH浓度分别为3.69±0.28mIU/ml、3.35±0.22mIU/ml,两组比较及与空白对照组(51.76±2.57pg/ml、3.44±0.20mIU/ml)相比,差异均无统计学意义;与CTX组(21.78±2.11pg/ml、6.24±0.22mIU/ml)比较,差异有统计学意义。OC保护组和GnRHa保护组的卵巢重量、卵泡数量与空白对照组相比,差异无统计学意义,明显高于CTX组,两组间比较差异亦无统计学意义。结论:GnRHa和口服避孕药均能减轻化疗药物对卵巢功能的损伤,从而保护卵巢储备功能。

Objective：To compare the effect of gonadotropin-releasing hormone agonist （GnRHa） and oral contraceptive （ OC, Diane-35 ） against cyclophosphamide （CTX） induced gonadotoxieity in female rats. Methods： The experiment consisted of 6 groups ： control group, CTX group, OC group, GnRHa group, OC protective group and GnRHa protective group, re- ceived normal saline, CTX, OC, GnRHa, OC ＋ CTX and GnRHa ＋ CTX respectively. Vaginal smear was used to judge the estrous cycle,the serum estradiol（ E2 ） and follicle-stimulating hormone（FSH） concentration was measured by radio-immuno assay, and respectively 5 rats were killed just the day, 15 days and 30 days after stopping medication to compare the weight of the ovary, the nmnbers of the follicle. Results： After 30 days, the serum estradiol in OC protective and GnRHa protective group were 51.33±2.00pg/ml,44.38±5.98pg/ml, the FSH concentra- tion in these groups were 3.69±0.28mIU/ml,3.35±0.22mIU/ml respectively. There was no statistical difference between the two groups, and between the two groups and control group. But there was significant difference between the two groups and CTX group. The ovarian weight and follicle numbers of OC protective and GnRHa protective group were similar to those of control group, but higher than those of CTX group. And there was no significant difference between the two groups. Conclusion：GnRHa and oral conceptive can both decrease the CTX-induced damage to the ovarian function, and protect the ovarian reserve function.