我国锡矿尘诱癌实验研究

The Study of Lung Cancer Induced in Rats by Tin Ore Dust

采用雄性3月龄Wistar 大鼠(体重180～220g)350只,分7组:(1)香花岭Ⅰ号矿尘组(含砷32.0%),6mg/次;(2)香花岭Ⅱ号矿尘(含砷2.2%),6mg/次;(3)香花岭Ⅲ号矿尘(含砷0.54%),6mg/次;(4)广西大厂锡矿选厂粉尘(含砷1.9%),6mg/次;(5)生理盐水对照组,0.2ml/次;(6)自然对照组;(7)3-MCA 阳性对照组,10mg,只注一次。每组50只大鼠。粉尘粒子直径在10μm 以下者占90%。大鼠乙醚麻醉,气管内灌0.2ml 生理盐水粉尘混悬液,每周一次,共10次。实验期限为二年。病理常规方法。每组诱发肺癌结果分别为:(1)2/38(5.0%);(2)2/48(4.0%);(3)2/46(4.0%);(4)0/42;(5)0/44;(6)4/31(12.9%)。结果表明各组肺癌诱发率与矿尘中的含砷量并无剂量-反应关系。

350 adult male Wister rats(body weight 180～200g)were used in the study.The rats were divided into seven groups:physiological saline as con- trol group,0.2ml/once;natural control group;3-MCA as poitive control group,10mg,only once,and four other experimental groups.There were fifty rats in each group.The diameter of ore dust less than 10μm account- ed for more than 90%.The rats were anesthesized by ethyl ether,and then 0.2ml physiological saline mixed with ore dust were instilled intratrache- ally once a week,for ten times totally.The stretch of experiment has been lasted for two years.Pathological routine examination was applied in the study.The result of lung cancer induced in each group were recorded res- pectively as follows:(1)2/38(5.0%);(2)2/48(4.0%);(3)2/46 (4.0%);(4)0/42;(5)0/44;(6)4/32(12.9%).The result shows that there is no dose-response relation between the incidence of lung can- cer of each group and of the content of arsenic in ore dust.