临床型3.0T MR在裸鼠原位胶质瘤模型确认及监测中的应用

Application of clinical 3.0T MR system in determination and monitoring of human glioma in situ model in nude mice

目的建立人源性裸鼠原位胶质瘤模型,探讨临床型3.0T MR对其进行成像的可行性和正确性。方法以U87MG细胞混悬液注入17只裸鼠脑实质,建立裸鼠原位胶质瘤模型。分别于接种后各时间点以临床型3.0T MR T1W、T2W及增强T1W扫描;扫描结束后处死荷瘤裸鼠,并行HE染色观察。于MRI及HE片上分别测量肿瘤最大截面积,并进行相关性分析。结果 14只裸鼠建模成功,T2WI及增强T1WI均可见明显肿瘤形成。接种后30天T2WI及增强T1WI测得的肿瘤最大截面积分别为(27.62±5.56)mm3、(28.80±5.42)mm3,均与HE测得结果[(25.65±4.95)mm3]成正相关(r=0.975、0.972,P均<0.01)。结论临床型3.0T MR能无创检测裸鼠的脑内成瘤情况,T2 WI及增强T1 WI均可很好地显示肿瘤,且能动态观察肿瘤生长情况。

Objective To establish a nude mouse model with intracephalic human glioma using a stereotactic inoculation,and to investigate the accuracy and feasibility of a clinical 3.0T MR scanner in evaluating and monitoring the growth characterizations of the intracephalic gliomas at different time points.Methods Seventeen mice were implanted for the model with intracephalic glioma using a stereotactic inoculation of human glioma U87MG cells.T2WI,T1WI and T1WI with contrast were obtained with a clinical 3.0T magnetic resonance after tumor transplantation.The animals were sacrificed after the last MR examination and were examined with HE staining.Max section areas of tumors in coronal view were measured in MRI and HE images,and the correlation was analyzed.Results The model was established successfully in 14 mice.T2WI and T1WI with contrast injection showed the gliomas distinctly in the brain of the mice,and the maximum area of the glioma was（27.62±5.56）mm3,（28.80±5.42）mm3 respectively 30 days after tumor transplantation,both were positively correlated with that of HE（[25.65±4.95]mm3,r=0.975,0.972,P〈0.01）.Conclusion Clinical 3.0T MR examination is an effective and non-invasive way to dynamically detect the occurrence and development of glioma in nude mice.T2WI and T1WI with contrast injection can show the tumors distinctly.