跑台运动训练对大鼠脑组织抗氧化能力的影响

Effect of treadmill exercises on antioxidant capacity in the brain of rats

目的:建立大鼠跑台运动模型,观察不同训练负荷对大鼠脑组织总抗氧化能力(T-AOC),过氧化氢酶(CAT),羟自由基(OH·)的影响。方法:建立有氧、无氧、有氧和无氧交替运动大鼠跑台运动训练模型,有氧运动时采用递增负荷训练,无氧运动时采用高速间歇训练,并设立正常对照组。各组大鼠训练结束后用机器匀浆法提取大鼠脑组织匀浆介质,紫外分光光度计检测大鼠脑组织T-AOC,CAT活性和OH·抑制能力。结果:有氧组大鼠OH·抑制能力要低于其他各组(P<0.05),但训练6周后,和其他组相比差异无显著性意义;交替组大鼠脑组织CAT活性要高于其他三组(P<0.05),而无氧组偏低(P<0.01);有氧运动组大鼠脑组织T-AOC要高于正常对照组(P<0.01)。结论:有氧运动的氧化损伤要强于其他各组,但是随着训练时间的延长,其抗氧化能力也随之加强。长期坚持适度的有氧运动将有利于机体提升抗氧化损伤的能力。

Objective：To establish a rat treadmill exercises model and to investigate the activity changes of total antioxidant capacity （T-AOC）, catalase （CAT） and hydroxyl free radical（OH·） in rat brain after treadmill exercises training of different intensity. Method：Eighty male SD rats were randomly divided into four groups：normal control group, aerobic exercises group （incremental load exercise）, anaerobic exercises group （high-speed intermittent training） and alterntive-training group aerobic and anaerobic alternative training. The activity of T-AOC, CAT and the inhibition ability of OH· were measured after every training period. Result：The inhibition ability of OH· of rat brain in aerobic exercise group was lower than that in the other groups （P0.05）. However, after six-week training, there was no significant difference between four groups. Activity of CAT rat brain in alternative-training group was higher than that in the other three groups （P0.05）, and it was low in anaerobic group （P0.01）. The T-AOC of rat brain in aerobic exercise group was higher compared with normal control group （P0.01）. Conclusion：Oxidative damages of aerobic exercise were heavier than the other groups. But with the extension of training period, the antioxidant capacity increased. It suggested that after moderate aerobic exercises for a long term organism＇s antioxidant activity would be enhanced.