系统性红斑狼疮患者循环annexinⅡ水平的变化及临床意义

Variation and clinical significance of circulation annexin H in patients with systemic lupus erythematosus

目的探讨系统性红斑狼疮（SLE）患者血白细胞annexinⅡ的表达水平及其在SLE中的临床意义。方法采用流式细胞术检测SLE患者35例，慢性肾炎患者10例，糖尿病肾病（DN）患者10例，健康对照组20名血白细胞annexinⅡ的表达水平，并分析其与临床指标间的相关性。采用t检验、方差分析及直线相关性分析进行统计学分析。结果SLE、DN组患者血白细胞annexinⅡ水平[（7．1±2．9）％，（8．0±3．7）％]均显著低于健康对照组[（10．6±1．6）％]（P〈0．01，P〈0．05）；有活动性[（SLE疾病活动指数（SLEDAI）评分≥9分]的SLE患者annexinⅡ水平[（5．6±2．4）％]显著低于非活动性（SLEDAI评分〈9分）的SIJE患者[（7．8±2．8）％]（P〈0.05）；SLE患者annexinⅡ水平与尿蛋白／肌酐呈负相关（r=-0．382，P〈0．05），与血白蛋白呈正相关（r=0，439,P〈0．01），与SLEDAI呈负相关（r=-0．417，P〈0．05），与D-二聚体呈负相关（r=-0．336，P〈0．05）。结论 SLE患者血白细胞annexinⅡ表达水平降低，参与了SLE患者高凝和继发性纤溶亢进状态的发生发展的病理生理过程，可作为一种早期反映SLE血栓前状态的良好指标，对判断SLE的活动性及疗效有一定的帮助。

Objective To compare the level of Annexin U in patients with systemic lupus erythematosus （SLE）, diabetic nephropathy （DN）, chronic glomerulonephritis and normal controls, and explore the significance of the annexin Ⅱ in SLE. Methods Thirty-five cases of patients with SLE, ten cases of patients with DN, ten eases of patients with chronic glomerulonephritis were enrolled in this study, twenty cases of healthy controls were also enrolled. Circulating annexin Ⅱ in white blood cells was detected by flow cytometry. Student＇s t test, variance, analysis and Linear correlation analysis were used for statisfial analysis. Results Compared with healthy controls, the level of annexin Ⅱ in white blood cells in SLE patients （7.1±2.9）% and DN patients （8.0±3.7）% were significantly lower than that of the healthy controls （P〈0.01, P〈0.05 ）. In the SLE group, the level of annexin Ⅱ of patients who had more active disease （SLEDAI ≥ 9 ） decreased more than those with less active disease（SLEDAI〈9）, （P〈0.05）. A positive correlation was found between annexin Ⅱ and serum albumin level （r=0.439, P〈0.01）, but negative correlation was found between annexia and urine protein/urine creatinine （r=-0.382, P〈0.05）, SLEDAI （r=-0.417, P〈0.05）, D-dimer （r=-0.336, P〈 0.05） levels. Conclusion The level of annexin Ⅱ is decreased in patients with SLE, so it can reflectthe abnormality of coagulation and fibrinolytic systems, and it may be used as a good indicator for prothrombotic status in SLE patients. It can he helpful to evaluatethe activity of the disease and the therapeutic efficacy.