第Ⅲ组代谢性谷氨酸受体抑制Aβ_(31～35)诱导的神经元胞内钙增高

Group Ⅲ mGluRs reduces intracellular calcium increased induced by Aβ_(31～35)

目的观察第Ⅲ组代谢型谷氨酸受体激动剂L-SOP和(R,S)-PPG对Aβ31～35所致培养神经元胞内Ca2+浓度升高的影响。方法原代培养的大鼠额叶皮层神经元,分别加入第Ⅲ组mG luR s激动剂L-SOP或(R,S)-PPG,利用实时Ca2+荧光成像技术观察对Aβ31～35所致〔Ca2+〕i升高的影响。结果急性给予Aβ31～35可引起培养神经元的〔Ca2+〕i水平呈剂量依赖性的升高,即随着Aβ31～35浓度的增加,其〔Ca2+〕i升高的幅度逐渐增加,而潜伏期逐渐缩短;经L-SOP或(R,S)-PPG预处理后,使Aβ31～35所引起的〔Ca2+〕i的平均升高幅度降低,平均潜伏期明显延长。结论在离体培养的神经元第Ⅲ组mG luR s的激活可通过抑制Aβ31～35引起的Ca2+超载,对Aβ31-35诱导的神经毒发挥保护作用。

Objective To observe the effect of group Ⅲ mGluRs agonist L-SOP and（R,S）-PPG on the increased level of intracellular calcium（（Ca2＋）i） of cultured neurons induced by Aβ31~35.Methods The cultured cortical neurons were pre-treated with L-SOP（100 μmol/L） or（R,S）-PPG（100 μmol/L） for 10 min.The（Ca2＋）i concentration of the cells were analyzed by real time fluorescence imaging technique.Results Aβ31~35（5,10,20,25 μmol/L） increased（Ca2＋）i level and decreased the average latency in a dose-dependent.The（Ca2＋）i increasing amplitude induced by Aβ31~35 was decreased after L-SOP or（R,S）-PPG pretreated,and average latency was longer.Conclusions Group Ⅲ mGluRs activation can preserve isolated culture neurons by inhibiting Ca2＋ overloading induced by Aβ31~35.