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Rac1 shRNA对卵巢癌细胞株Skov3生物学行为的影响 被引量：1

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摘要 目的为Rac1 shRNA治疗卵巢癌提供实验依据。方法 Rac1 shRNA载体pGPU6/GFP/Rac1-524以L ipofectim ineTM2000介导转染卵巢癌细胞株Skov3,Transwell肿瘤细胞迁移和侵袭实验检测转染细胞的迁移和侵袭能力,W estern印迹分析转染细胞MMP2蛋白的表达情况。结果转染Rac1 shRNA载体pGPU6/GFP/Rac1-524组、转染非特异shRNA载体pGPU6/GFP/NC组和未转染组迁移到滤膜底面的细胞数分别为97±21、319±75、365±92,而侵袭到滤膜底面的细胞数分别为79±22、285±63、324±84,转染Rac1 shRNA载体组与转染pGPU6/GFP/NC组、未转染组比较差异显著(P<0.05),转染pGPU6/GFP/NC组与未转染组相比无统计学意义(P>0.05)。转染Rac1 shRNA载体pGPU6/GFP/Rac1-524组MMP2蛋白的表达量下降。结论 Rac1 shRNA载体pGPU6/GFP/Rac1-524可有效抑制卵巢癌细胞Skov3的迁移和侵袭,该抑制作用可能是通过调控MMP2的表达而实现的。
作者 王虹 张菊青 陈玉丙 张红梅 崔满华
机构地区 北华大学附属医院妇产科 吉林大学第二医院 吉林大学中日联谊医院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2011年第9期1601-1603,共3页 Chinese Journal of Gerontology
基金 吉林省自然科学基金项目(201015175)
关键词 RACL RNA干扰 卵巢癌 肿瘤细胞迁移和侵袭
分类号 R737 [医药卫生—肿瘤]
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参考文献11

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共引文献6

同被引文献15

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二级引证文献1

中国老年学杂志
2011年 第9期

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