冷胁迫条件下拟南芥Rubisco相互作用蛋白质的比较分析(英文)

Comparative Profile of Rubisco-interactingProteins From Arabidopsis: PhotosynthesisUnder Cold Conditions

1,5-二磷酸核酮糖羧化酶/加氧酶(Rubisco,EC 4.1.1.39)在生物适应环境变化的过程中起到重要的作用.位于叶绿体中与冷胁迫密切相关的非常重要的复合酶——Rubisco,其相互作用的蛋白质至今没有系统的研究.对拟南芥进行4种处理:a.持续在20℃生长(对照);b.4℃4 h冷胁迫;c.4℃24 h冷胁迫;d.4℃24 h冷胁迫后放入20℃恢复24 h.然后利用免疫共沉淀、十二烷基硫酸钠聚丙烯酰胺凝胶电泳及基质辅助激光解析电离飞行时间质谱技术,在冷胁迫条件下研究了拟南芥光合抑制与Rubisco相互作用蛋白质解聚之间的关系.在鉴定出的5个与冷胁迫相关的Rubisco相互作用蛋白质中,AAA-型ATP酶家族蛋白和糖基转移酶对Rubisco活性及植物适应冷胁迫起着重要的作用.研究结果表明,Rubisco复合酶体系的解聚可能是低温胁迫下拟南芥光合速率降低的主要原因.

Rubisco （Ribulose 1, 5-bisphosphate carboxylase/oxygenase, EC 4.1.1.39） is crucial in biological circumstance fluctuation.Although disassembly of Rubisco after chill treatment has been reported in previous studies, there is only little known data on Rubiscointeractive proteins involved in the disassembly process of Rubisco. Both repression of net photosynthesis rate and disassemblyof Rubisco large subunits （Ru-L） have been investigated in the wild type, Arabidopsis thaliana （Col-0）, treated at 4℃ for 4 h and24 h together with their 24 h recoveries at 20℃. Co-immunoprecipitation coupled with sodium dodecyl sulfate polyacrylamide gelelectrophoresis （SDS-PAGE） analysis and MALDI-TOF MS identification was used to explore Rubisco-interacting proteins. Fiveprotein candidates were profiled. The identified AAA-type ATPase family and glycosyltransferase were determined crucial for Rubiscoactivity. It is also strongly correlated to cold acclimation. Results suggest that the disassembly of Rubisco might have been the maincause of photosynthesis rate reduction under chill conditions, rather than photosystem or biogenesis involvement.