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小儿肾母细胞瘤中SOCS-1和SOCS-3的表达及其临床意义 被引量：1

Expressions and clinical significance of SOCS-1 and SOCS-3 in Nephroblastoma
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摘要 目的 探讨小儿肾母细胞瘤中细胞因子信号转导抑制因子-1（SOCS-1）和细胞因子信号转导抑制因子-3（SOCS-3）的表达,以及与小儿肾母细胞瘤不同病理类型之间的内在关系.方法 应用RT-PCR法及Western-Blot法测定10例小儿正常肾脏组织、30例小儿肾母细胞瘤及瘤旁组织中sOCS-1和SOCS-3的表达.结果 与正常小儿肾脏组织（mRNA表达：0.721±0.183、0.694±0.148,蛋白表达：0.746±0.168、0.652±0.183）相比,肾母细胞瘤组织中SOCS-1和SOCS-3 mRNA表达（0.156±0.027、0.103±0.021）及蛋白的表达（0.205±0.079、0.185±0.041）均明显降低（P＜0.01）.在肿瘤不同病理类型之间SOCS-1和SOCS-3mRNA的表达（预后良好型：0.175±0.086、0.149±0.100,预后不良型：0.128±0.074、0.091±0.067）有差异（P＜0.05）.瘤旁组织SOCS-1 和SOCS-3mRNA的表达（0.572±0.154、0.315±0.137）及蛋白的表达（0.628±0.183、0.439±0.137）与正常肾脏组织有差异（P＜0.05）.结论 SOCS-1和SOCS-3的表达下降可能与小儿肾母细胞瘤的发生、发展密切相关,与病理分期也有一定联系. Objective To investigate the expression levels of suppressor of cytokine signaling-1 and suppressor of cytokine signaling-3 in the Wilms＇ tumor and to study the inherent relationship between the different pathological types. Methods The expression levels of SOCS-1 and SOCS-3 were assessed by RT-PCR and Western-Blot in ten normal kidney tissues, thirty nephroblastoma specimens and adjacent tissues. Results Compared to the normal kidney tissues（mRNA expressions： 0.721±0.183、0.694±0.148,protein expressions：0.746±0.168、0.652±0.183）, the expressions of mRNA （0.156±0.027、0.103±0.021）and protein（0.205±0.079、0.185±0.041）of the SOCS-1 and SOCS-3 in nephroblastoma were significantly lower （P〈0.01）. There were statistically significant between mRNA expressions and the different pathological types（FH：0.175±0.086,0、149±0.100,UH：0. 128±0.074、0.091±0.067） （P〈0.05）.The difference betweenneuroblastoma and the normal tissues in expressions of mRNA （0. 572 ± 0. 154、0. 315 ± 0. 137）and protein（0. 628± 0. 183、0. 439 ± 0. 137）of the SOCS-1 and SOCS-3 were statistically significant （P〈0. 05）. Conclusions The low SOCS-1 and SOCS-3 expressions may have a role in the development and progress of nephroblastoma, and the different pathological types.
作者 陈志鸿 于启海 许晓群 吴荣德 刘娣 潘奇 崔明宇
机构地区 山东大学附属省立医院小儿外科 山东省科技重点实验室
出处 《中华小儿外科杂志》 CSCD 北大核心 2011年第5期321-325,共5页 Chinese Journal of Pediatric Surgery
基金 基金项目：本课题受山东省自然科学基金资助（编号：Y2006C134）
关键词 肾母细胞瘤 细胞因子信号转导抑制因子-1 细胞因子信号转导抑制因子-3 Nephroblastoma SOCS-1 SOCS-3
分类号 R737 [医药卫生—肿瘤]
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参考文献18

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二级参考文献19

  • 1Zhang JG, Metealf D, Rakar S, et al. The SOCS box of suppressor of cytokine signaling-1 is important for inhibition of cytokine action in vivo[J]. Proc Natl Acad Sci USA, 2001,98(23) :13261-13265.
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  • 8Metcalf D, Mifsud S, Di Rago L, et al. Polycystic kidneys and chronic inflammatory lesions are the delayed consequences of loss of the suppressor of cytokine signaling-1 ( SOCS-1 ) [ J ]. Proc Natl Acad Sci USA, 2002, 99(2) :943-948.
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共引文献7

同被引文献7

  • 1Weber A, Hengge UR, Bardenheuer W? et al. SOCS-3 is frepuentlymethylated in head and neck squamous cell carcinoma and its precursorlesions and causes growth inhibition[J], Oncogene,2005,24(44) :6699-6708.
  • 2Zhou H,Miki R, Eeva M, et al. Reciprocal regulation of SOCS1 andSOCS3 enhances resistance to ionizing radiation in glioblastoma multi-form[J]. Clin Cancer Res,2007,13(8) :2344-2353.
  • 3Niwa Y, Kanda H, Shikauchi Y, et al. Methylation silencing ofSOCS-3 promotes cell growth and migration by enhancing JAK/ST AT and FAK signalings in human hepatocelluar carcinoma[J]. Oncogene,2005,24(42) :6406-6417.
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  • 6曹军,欧阳艳.肝癌组织中SOCS-3mRNA的表达[J].现代生物医学进展,2009,9(8):1499-1500. 被引量：2
  • 7吴庆宇,陈珂珂,高龙,张伟,倪克樑.SOCS-3基因在结肠癌中的表达及其甲基化测定[J].胃肠病学和肝病学杂志,2010,19(7):608-611. 被引量：2

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中华小儿外科杂志
2011年 第5期

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