D4S1627、D14S1426和D1S1649 STR基因座的复合扩增同步检测及遗传多态性分析

Multiplex PCR and Genetic Polymorphism of STR Loci D4S1627,D14S1426,D1S1649

目的制备D4S1627、D14S1426、D1S1649 STR基因座的等位基因分型标准物,构建这3个STR基因座的复合扩增同步检测方法并进行遗传多态性分析。方法本实验采用复合扩增同步检测方法,聚丙烯酰胺凝胶电泳分离,银染显色,检测成都地区120名无关汉族个体的D4S1627、D14S1426、D1S1649 STR基因座基因型及等位基因频率分布。结果 3个STR基因座复合扩增同步检测电泳分型条带清晰,各等位基因片段互不重叠,每一个体样本与单个基因座检测结果一致。复合扩增同步检测方法结果显示,3个STR基因座在成都市汉族群体中的基因型分布符合Hardy-Weinberg平衡,基因座D4S1627检出6个等位基因(10～15);D14S1426检出9个等位基因(5、6、8～14),未检出等位基因7;D1S1649检出6个等位基因(7～12)。3个STR基因座的累积个人识别率为0.9989,累积非父排除率为0.9603。结论成功建立D4S1627、D14S1426和D1S1649 STR基因座复合扩增同步检测方法,这3个STR基因座在群体遗传研究以及法医学鉴定中具有实际应用价值。

Objective To prepare the allelic ladder materials of three STR loci includes D1S1649,D4S1627,D14S1426,and construct a synchronous detection method by multiplex amplification of the three loci.Methods Multiplex PCR method,PAGE（polyacrylamide gel electrophoresis） and silver staining were applied to detect 120 unrelated Chinese Han population from Chengdu whose allele and gene frequency distributing of these three loci.The electrophoretic bands of the multiplex PCR of these three loci were clear,no overlap of each alleles were observed,and the results were the same with single-locus test results.The results of the multiplex PCR demonstrated that the genotype of these three STR loci in Chengdu Han population in the genotype distribution met with Hardy-Weinberg equilibrium.6 alleles（10-15） were detected in D4S1627,9 alleles（5,6,8-14,without 7） were detected in D14S1426,6 alleles（7-12） were detected in D1S1649.Conclusion The results suggested that the multiplex PCR system of these three loci is of practical value in genetic research and forensic identification.