理脾调脂胶囊对血脂失调症大鼠及ApoE基因敲除小鼠PPARs mRNA的调控作用

Regulatory Effect of Lipi Tiaozhi Capsule on the Expression of Peroxisome Proliferator-activated Receptors mRNA in Dyslipidemia Rats and ApoE^(-/-)Mice

目的研究理脾调脂胶囊对血脂失调症大鼠及ApoE基因敲除(ApoE-/-)小鼠PPARα、γmRNA的调控作用,探讨其调节脂质代谢的作用机制。方法 48只Wistar大鼠随机分为空白组、模型组、理脾调脂胶囊治疗组(理脾调脂组)、血脂康对照组(血脂康组),造模4周,将ApoE-/-小鼠30只随机分为空白组、血脂康组、理脾调脂组。理脾调脂组大鼠、ApoE-/-小鼠(以下简称大、小鼠)每日灌胃理脾调脂胶囊,血脂康组每日灌胃血脂康,用药8周。酶法测定大、小鼠血清总胆固醇(TC)、甘油三酯(TG)浓度,沉淀法测定大、小鼠血清高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C);内参照模板法荧光定量PCR检测大、小鼠肝脏PPARα、γmRNA的表达。结果理脾调脂组大、小鼠血清TC、TG、LDL-C浓度较模型组、空白组显著降低(P<0.01),HDL-C浓度显著提高(P<0.01),大、小鼠PPARα、γmRNA表达较模型组(或空白组)显著提高(P<0.01);与血脂康组比较,理脾调脂组大、小鼠血清TC、TG、LDL-C浓度均降低(P<0.05),HDL-C浓度均显著提高(P<0.01,P<0.05),PPARα、γmRNA表达明显提高(P<0.05)。结论理脾调脂胶囊能显著提高实验性血脂失调症大、小鼠肝脏PPARα、γmRNA的表达,具有调控核因子而影响血脂代谢的作用。

Objective To study the regulatory effect of Lipi Tiaozhi Capsule （LTC） on the expression of peroxisome proliferator-activated receptor （PPAR） a and y mRNA in dyslipidemia rats and ApoE-/- mice, and to explore its mechanisms for regulating lipid metabolism. Methods 48 Wistar rats were randomly divided into the blank group, the model group, the treatment group （treated by LTC）, and the control group （treated by Xuezhi- kang Capsule）. After four-week modeling （except the blank group） 30 ApoE-/- mice were randomly divided into the blank group, the treatment group, and the control group. LTC was given by gastrogavage to rats and ApoE-/- mice in LTC groups while XZKC was given to XZKC groups. The medication was conducted once daily for eight weeks. The serum TC and TG contents of rats and mice were determined by enzymic method. The ser- um high-density lipoprotein cholesterol （HDL-C） and low-density lipoprotein cholesterol （LDL-C） were detected by precipitation method. PPARα and y mRNA expressions were detected in the liver tissue of the rats and mice by fluorescent PCR. Results Compared with the model group and the blank group, the serum contents of TC, TG, and LDL-C of rats or mice in the treatment group decreased significantly （P〈0.01）. The serum content of HDL-C increased significantly （P 〈0.01 ）. PPARa and y mRNA expressions of rats or mice increased significant- ly （P〈0.01）. Compared with the control group, the serum contents of TC, TG, and LDL-C of mice and rats in the treatment group decreased （ all P 〈 0.05）, the serum content of HDL-C increased significantly （ P 〈 0.05, P 〈0.01 ）. And PPARα and ）, mRNA expressions of rats or mice increased significantly （P 〈0. 05）. Conclusion LTC could significantly increase PPARa and y mRNA expressions of experimental dyslipidemia rats and ApoE-/- mice, playing roles in regulating nuclear factors and further effecting lipid metabolism.