摘要
目的研究人血管生成素1(Ang-1)、血管内皮生长因子165(VEGF165)以及两种因子相互作用对人胃癌细胞株MGC-803增殖与凋亡的影响。方法应用MTT法测定腺病毒绿色荧光蛋白(Ad-GFP)(B组)、Ad-Ang-1(C组)、Ad-VEGF165(D组)以及Ad-Ang-1+Ad-VEGF165/2(E组)对MGC-803细胞增殖的影响;另设对照组(A组)。采用流式细胞术分析血清饥饿时其对凋亡的影响;运用Western blot方法检测Bcl-2和Bax蛋白的表达。结果 24、48、72 h时,C、D、E组吸光度均明显高于B、A组(P<0.05);E组吸光度明显高于C、D组(P<0.05)。与A组或B组相比,C、D、E组均能够抑制细胞凋亡(P<0.01),其中E组抑制凋亡作用最强。C、D、E组Bcl-2蛋白表达均比B、A组明显增高(P<0.05),Bax蛋白的表达则明显降低(P<0.05)。结论 Ang-1、VEGF165和Ang-1+VEGF165/2能够明显促进MGC-803细胞体外增殖,抑制血清饥饿时的凋亡;Ang-1与VEGF165联合作用要显著强于单用Ang-1或VEGF165。
Objective To explore the effects of angiopoietin-l(Ang-1) and vascular endothelial growth factor (VEGFI65) on the proliferation and apotosis of human gastric cancer cell line MGC-803. Methods MGC-803 was transfected with Ad-GFP (group B), Ad-Ang-1 (group C), Ad-VEGF165(group D) or Ad-Ang-1 plus Ad-VEGF165 (group E). MGC-803 in group A was blank control. The proliferation and apoptosis of MC, C-803 were measured by MTT colorimetry assay and flow counting, respectively. Expressions of Bcl-2 and Bax proteins were measured by Western blot. Results Proliferation of MCK2-803 in groups of C, D, E at 24,48 and 72 h were higher than that in groups of B and C(P〈0.05), which was higher in group E than that in groups of C and D(P〈O. 05). Compared to groups of B and A, cell apoptosis was significantly inhibited in groups of C, D, E, especially in group E, (P〈0. 01). Expression of Bcl-2 was increased, but that of Bax decreased, in groups of C,D,E than that in groups of B and A(P〈0.05). Conclusion Ang-1 and VEGF165 can significantly promote the proliferation of MGC-803 and reduce its apoptosis. A combination of Ang-1 and VEGF165 has stronger effect on MGC-803 than Ang-l or VEGF165 alone.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第8期876-879,共4页
Jiangsu Medical Journal