冠心病与MBL基因多态性及血浆MBL和hs-CRP水平的相关性

Correlation of gene polymorphism and plasma contents of MBL and hs-CRP in patients with coronary heart disease

目的研究冠心病(CHD)与甘露聚糖结合凝集素(MBL)基因多态性及其血浆MBL和高敏C-反应蛋白(hs-CRP)水平的关系。方法收集100例CHD患者(CHD组)和60例健康人群(对照组)抗凝血,限制片段长度多态性法(PCR-RFLP)检测MBL ExonI 54位密码子基因点突变,ELISA法和免疫比浊法分别检测血浆MBL含量和高敏C-反应蛋白(hs-CRP)水平。结果 CHD组与对照组MBL基因ExonI 54位密码子点突变频率比较差异无统计学意义(P>0.05)。CHD组血浆MBL和hs-CRP含量均高于对照组(P<0.01)。而CHD组内血浆hs-CRP水平升高者(>3 mg/L)与hs-CRP水平正常者(<3 mg/L)MBL含量比较差异无统计学意义(P>0.05)。结论 MBL和hs-CRP可能以不同机制参与CHD发生、发展过程。

Objective To analyze the correlation between coronary heart disease（CHD） and the gene polymorphism of mannan binding lectin（MBL）, the plasma contents of MBL and the level of high-sensitivity C-reactive protein（hs-CRP）. Methods The anti-coagulated blood of CHD patients （group CHD, 100 cases） and healthy people（group C, 60 cases） was collected. The point mutation of MBL ExonI 54 codon was detected by PCR-RFLP. The content of MBL and the level of hs-CRP were detected by ELISA kit and immunoturbidimetry, respectively. Results There was no significant difference in the genotypes of MJ3L ExonI 54 codon between group CHD and group C（P〉0. 05）. The plasma content of MBL and the level of hs-CRP in group CHD were higher than those in group C （P〈0.01）. In group CHD, there was no obvious difference in the content of MBL between the patients with high level of hs-CRP（〉3 mg/L） and those with normal level of hs-CRP（〈3 mg/L） （P〉0. 05）. Conclusion MBL and hs-CRP may be involved in the occurrence and development of CHD via different mechanisms.