四种ELISA方法检测食品中黄曲霉毒素B1的对比研究

Comparative Study of Four ELISA Methods for the Determination of Aflatoxin B_1 in Foods

继李秀芳等(1987年)建立反向直接竞争ELISA法测定黄曲霉毒素B_1(AFB_1)之后,本文作者又建立了直接竞争ELISA、间接竞争ELISA 及生物素-亲合素ELISA(BA-ELISA)三种检测食品中黄曲霉毒素B_1的方法。并将此四种方法进行了比较。另外,将ELISA方法与其它AFB_1检测方法(TLC、RIA、HPLC)进行了对比研究。结果表明直接竞争ELISA及间接竞争ELISA具有灵敏、特异、简便、快速、安全、价廉等特点,适合在我国基层推广应用。

Direct,reversed direct,indirect and BA-ELISA methods were develo- ped for determining aflatoxin B_1 in foods.The comparison among four ELISA methods and the ELISA methods with TLC,RIA,HPLC showed that direct and indirect ELISA were simple,sensitive,specific,rapid and economical.These two methods can be used in county food hygiene labora- tories in our country.