痰热清抗呼吸道合胞病毒作用体外实验研究

Effect of Tanreqing injection on anti-respiratory syncytial virus(RSV,long strain) in vitro

目的研究中草药注射液痰热清对呼吸道合胞病毒(RSV、Long株)的抑制作用。方法以病毒唑为阳性对照药,采用细胞培养技术,观察不同药物浓度及不同给药方式下RSV攻击后各组Hep-2细胞的病变效应(CPE),在此基础上采用MTT比色法,测定各组细胞的病毒抑制率。以CPE法计算药物的半数中毒浓度TC50,分别以CPE法及MTT法计算药物的半数有效浓度EC50及治疗指数TI,比较不同药物浓度及不同给药方式下热毒宁对呼吸道合胞病毒(RSV)的抑制作用效果。结果痰热清注射液半数中毒浓度TC50为3.972g/L,预防给药方式、细胞外直接灭活及治疗给药方式均有抗RSV作用,其抗RSV的半数有效浓度(EC50)分别为0.428/1.160/1.189g/L,治疗指数TI分别是9.28、3.42和3.34,痰热清对RSV的抑制作用存在着明显的量效反应关系。结论痰热清对RSV有直接灭活作用,对RSV侵入Hep-2细胞有阻断作用,对RSV在Hep-2细胞内增殖有抑制作用,相同浓度下,以预防作用更显著。

Objective To explore anti-respiratory syncytial virus（RSV） effect of Chinese medicinal Tanreqing injection.Methods The technique of cell cultuer and MTT with ribavirin as positive concentration-dependent manner.Results The median toxic concentration（TC50） of 3.972 g/L in Hep-2 cell culture.The median effective concentration（EC50）was 0.428 g/L when drug was added 24h before infection,the EC50 was 1.160g/L when drug was added 2 h after virus infection,and it was 1.189 g/L as the drug was added after it mixed with virus 24 h.The therapeutic index（TI）was 9.28,3.42and 3.34 respectively.Conclusions Tanreqing injection has the direct deactivation and blocking effect on RSV.The preventive effect is the most evident with the same concentration in vitro.