HPLC测定小儿消积止咳口服液中辛弗林、氢溴酸槟榔碱的含量

Determination of synephrine and arecoline contents in Xiao′er Xiaoji Zhike oral liquid

目的：建立HPLC测定小儿消积止咳口服液中辛弗林、氢溴酸槟榔碱含量的方法。方法：Symmetry C18色谱柱（4.6 mm×250 mm;5μm）,流动相乙腈-甲醇-0.1%冰醋酸溶液（每1 000 mL含磷酸二氢钾0.6 g,十二烷基磺酸钠1.0 g）（15∶30∶55）,检测波长215 nm。结果：辛弗林、氢溴酸槟榔碱分别在0.281 52.815,0.040 160.401 6μg呈良好的线性关系,回收率分别为97.1%（RSD 1.4%）,100%（RSD 1.3%）。结论：该法简便、测定准确、灵敏度高,分离效果好,能用于该品种的质量评价。

Objective： To develop a HPLC method for the determination of synephrine and arecoline contents in Xiao′er Xiaoji Zhike oral liquid.Method： The analysis was performed on a Symmetry C18 column（4.6 mm×250 mm,5 μm） eluted with acetonitrile-methanol-0.1% acetic acid solution（containing potassium dihydrogen phosphate 0.6 g and SDS 1.0 g per 1 000 mL）（15∶30∶55）as mobile phase.The detection wavelength was set at 215 nm.Result： The synephrine and arecoline concentrations had good linear relationship between 0.281 5-2.815 and 0.040 16-0.401 6 μg（r〉0.999 7）,and the average recoveries of the two compounds were 97.1%（RSD 1.4%） and 100%（RSD 1.3%）,respectively.Conclusion： The method is simple,accurate and sensitive.