儿童急性白血病微小残留病检测方法与评价

Assays of the minimal residual disease in pediatric acute leukemia

白血病的微小残留病常成为白血病复发的根源,定量检测微小残留病对指导临床治疗具有重要意义.目前常用的定量检测方法有三种,包括检测异常抗原表型的流式细胞技术、抗原受体基因重排的PCR扩增技术、融合基因的PCR扩增技术.每种方法原理及特点不同导致I临床应用范围不同.根据患者自身特点,选择合适的检测方法,能使微小残留病定量更准确.

Minimal residual disease（MRD）iS the origin of relapse in leukemia.So it＇s significant to quantitate MRD in clinical.At present, there are three MRD quantitative assays, such as flow cytometric detection of abnormal immuno-phenotype,polymerase chain reaction（PCR）amplification of antigenreceptor genes and fusion transcripts.Because every measure has its own principle and feature.the clinical applications are different.Selecting suitable assays based on the characteristic of patients can help to quantitate MRD exactly.