湿地松松节油标准样品的制备与定值研究

Preparation and Certification of Reference Turpentine Oil Sample from Pinus elliottii Engelm.

研究了湿地松松节油标准样品的制备和稳定性、均匀性及其定值方法。湿地松松节油制备采用水蒸气蒸馏法,含量分析采用气相色谱面积归一化法。制备得到松节油标准样品在36个月内保持稳定,通过了均匀性检验。多实验室联合定值结果为:α-蒎烯含量X标=62.41%,标准不确定度UA=0.15%,扩展不确定度U0.95=0.30%;β-蒎烯含量X'标=31.66%,标准不确定度U'A=0.09%,扩展不确定度U'0.95=0.18%;β-水芹烯含量X″标=3.33%,标准不确定度U″A=0.05%,扩展不确定度U″0.95=0.10%。

The reference turpentine oil sample from Pinus elliottii Engelm. （slash pine） was prepared, and their homogeneity, stability, and method of its certification were studied. The turpentine oil was prepared through steam distillation from slash pine oleoresin, and measured by a gas chromatographic area normalization. The reference turpentine oil from P. eUiottii Engelm. has passed the test of homogeneity, and it has kept tobe stable for at least 36 months. The certification results of the reference sample through multiple lab＇s co-works showed that the standard content values of 3 index components were： α-pinene 62.41% with standard uncertainty UA =0. 15 % , extended uncertainty U0.95 = 0. 30 % ; β-pinene 31.66 % with standard uncertainty U＇A = 0. 09 % , and extended uncertainty U＇0.95 =0.18 % ;β-phellandrene 3.33 % with standard uncertainty U＇A =0.05 % , extended uncertainty UA.95 =0.10 %, respectively.