江苏省胃癌高发区幽门螺杆菌感染与胃癌、基因突变之间的关系

The relationship between Helicobacter pylori infection, gene mutation and gastric carcinoma in gastric cancer prevalent areas of Jiangsu Province

目的研究江苏省胃癌高发区幽门螺杆菌（Ｈｐ）感染及其毒力因子与胃癌、基因突变之间的关系。方法用酶链免疫吸附测定（ＥＬＩＳＡ）、免疫印迹及聚合酶链反应单链构象多态性分析（ＰＣＲＳＳＣＰ）等方法，分析了５０例胃癌及５０例配对胃炎患者的Ｈｐ感染、Ｈｐ的细胞毒素相关蛋白（ＣａｇＡ）、空泡细胞毒素（ＶａｃＡ）情况及胃粘膜ｐ５３基因第５～８外显子的突变。结果胃癌组中Ｈｐ阳性率及ＣａｇＡ阳性率均显著高于胃炎组，ｐ５３基因突变与Ｈｐ感染有非常显著的相关性，而与ＣａｇＡ、ＶａｃＡ的表达无关。结论Ｈｐ（尤其是ＣａｇＡ阳性菌株）感染能增加胃癌的发病风险，在Ｈｐ致癌过程中ｐ５３等基因突变可能起了关键的作用，研究结果为根治Ｈｐ以预防胃癌发生提供了实验依据。

Objective To investigate the relationship botween Hp(Helicobacter pylori) infection and gene mutation, in gastric carcinoma prevalent areas of Jiangsu Province. Methods We studied the prevalence of Hp infection, the status of CagA and VacA of Hp, and p53 exon 5-8 mutations in tissues of 50 gastric carcinoma patients and 50 gastritis patients by the methods of PCRSSCP, immunoblotting and ELISA. Results The positive rates of Hp infection and CagA expression in gastric carcinoma group were significantly higher than those in control group. p53 mutation was significantly associated with Hp infection, but not with the CagA and VacA expression. Conclusions Hp infection, especially the CagA positive strain, increases the risk of gastric carcinoma, in which p53 mutation may play an important role. The above results suggest that eradication of Hp might prevent the occurrence of gastric carcinoma.