土壤甲烷单加氧酶活性测定的研究

Study on the Determination of Soil Mathane Monooxygenase Activity

以垃圾生物覆盖土、垃圾填埋场覆盖土、菜地土为材料,研究了土壤甲烷单加氧酶(Methane monooxygenase,MMO)活性的测定条件。结果表明:0.04 g kg-1 NaN3含量可抑制土壤生物活性,可作为土壤MMO活性测定时的对照;最适的底物丙烯浓度可为0.5%;培养时间对土壤MMO活性测定影响不大,但从测定的误差和可靠性考虑,可选用5 d作为土壤MMO活性测定的培养时间。统计分析表明:采用该方法测定的土壤MMO活性与其甲烷氧化潜力有很好的线性关系。

The conditions for measuring soil methane monooxygenase（MMO） activity were studied using waste biocover soil,landfill cover soil and vegetable soil.The results showed that 0.04 g kg^-1 NaN3 inhibited soil biological activity,which could be used as control for the analysis of MMO activity.0.5% propylene was a suitable substrate concentration.The incubation time didnt have an obvious effect on the measurement of soil MMO activity,but as far as measurement deviation and reliability were concerned,5 days＇ incubation was the optimum time.Statistical analysis showed that a strong linear relationship was observed between soil MMO activity and methane oxidation potential.