香菇多糖对溃疡性结肠炎大鼠肠道微生态失调的调整作用研究

The regulatory effect of Lentinan on dysbacteria in rats with ulcerative colitis

目的研究香菇多糖通过扶植肠道正常菌群生长,控制内毒素易位,对溃疡性结肠炎大鼠肠道微生态失调进行调整,从微生态学角度防治溃疡性结肠炎。方法 Wistar大白鼠48只,随机取8只作为正常对照组,其余40只造模。之后以香菇多糖和丽珠肠乐灌胃,14 d后处死。取肠内容物、血液,分别进行细菌培养、检测挥发性脂肪酸、内毒素。结果经过香菇多糖和丽珠肠乐治疗后,大鼠肠道双歧杆菌、乳酸杆菌的数量明显上升,大肠埃希菌、肠球菌的数量明显下降;肠内容物挥发性脂肪酸含量显著上升;血中内毒素含量显著下降。结论香菇多糖和丽珠肠乐均具有扶植正常菌群生长,调整菌群失调,提高机体免疫力,防治溃疡性结肠炎的作用;香菇多糖和丽珠肠乐联合应用效果更佳。

Objective To study the effect of lentinan on dysbacteria in rats with ulcerative colitis.Method From 48 Wistar rats,8 were randomly selected as the normal control group,and the remaining 40 were used to establish the models. The rats were given lentinan and Lizhu Chang Le（live bifidobacterium preparation） via gastric gavage.After 14 days the intestinal contents and blood were cultured for bacteria,volatile fatty acids and endotoxin detection.Result After treatment with lentinan and Lizhu Chang Le,the number of Bifidobacterium and Lactobacillus increased,while the number of Enterococci decreased;Volatile fatty acids in intestinal contents increased significantly and blood endotoxin decreased significantly.Conclusion Lentinan and Lizhu Chang Le can foster the growth of normal intestinal flora,adjust the balance of flora and improve immunity,thus prevent ulcerative colitis.Lentinan combined with Lizhu Chang Le will give better result.