辛二酰苯胺异羟肟酸(SAHA)对乳腺癌细胞增殖影响的动物实验研究

Effect of Histone Deacetylase Inhibitor SAHA on Breast Cancer Cell in Animal Mode

目的探讨组蛋白去乙酰化酶抑制剂SAHA对乳腺癌细胞株增殖周期的影响。方法饲养Balb/c-nu/nu雌性裸鼠,细胞悬液皮下注射法构建人乳腺癌细胞株MCF-7裸鼠移植瘤模型,随机分为6组,经尾静脉注射不同剂量的SAHA,获取血细胞分析、血脂、肝功能、肾功能、裸鼠体重、瘤重等数据,计算肿瘤生长抑制率、移植瘤的体积,进行统计分析。结果不同剂量的SAHA作用于乳腺癌荷瘤裸鼠后,通过比较肿瘤体积及抑瘤率均显示出疗效,在0.10~0.42 mg/kg剂量范围内,SAHA的治疗效应呈现剂量依赖关系;其作用后导致血清胆红素和谷丙转氨酶水平升高,对红细胞、白细胞及血小板计数、尿素氮、肌酐水平影响不明显。结论 SAHA是乳腺癌细胞增殖抑制剂,其抑制效应有一定的量效关系;其对机体副作用较小。

Objective To study the effect of histone deacetylase inhibitor SAHA on breast cancer cell in animal mode.Methods MCF-7 breast cancer nude mouse model was constructed by hypodermic injection of breast cancer cell suspension,and different dose of SAHA was given through tail vein injection.The blood was then taken to analyze the blood fat,the liver and the kidney function,and the body weight and wet weight of tumor were measured.The tumor growth inhibition rate and tumor volume were analyzed by SPSS 10.0.Results Curative effect was observed in all the dose level group of SAHA.Within the 0.10~0.42 mg/kg dosage range,the SAHA treatment effect presented the dose-dependent relations,but no additional curative effect was observed when the dose increased from 0.42 mg/kg to 0.84 mg/kg;Increase of serum glutamic-pyruvic transaminase and bilirubin was observed after SAHA treatment,but SAHA had no effects on the kidney function,blood count and cholesterol.Conclusion SAHA is a powerful and dose-dependant histone deacetylase inhibitor for breast cancer cell with few side effects.