磁扭力刺激人肺微血管内皮细胞模型的建立

Establishment of the in vitro model of human pulmonary microvascular endothelial cells stretched by magnetic twisting stimulation

目的观察人肺微血管内皮细胞在磁扭力刺激后肌动蛋白骨架的变化。方法人工合成整合素胞外保守肽段序列精氨酸-甘氨酸-天冬氨酸(Arg-Gly-Asp,RGD)包被磁珠与人肺微血管内皮细胞孵育2 h后,放入磁扭力刺激仪,通过磁珠的运动牵拉细胞2 h,荧光探针鬼笔环肽(FITC-phalloidine)染色肌动蛋白纤维。结果磁珠与细胞共孵育2 h后,两者紧密结合;磁扭力刺激后应力纤维形成。结论磁扭力刺激人肺微血管内皮细胞模型是实施细胞牵拉的理想模型。

Objective To investigate the morphological changes of actin cytoskeleton in humanpulmonary microvascular endothelial cells exposed to magnetic twisting stimulation. Methods Ferromagnetic beads were mated with artificial synthesized extracellular peptide of integrin as Arg-Gly-Asp （RGD）, and then incubated with human pulmonary microvascular endothelial cells for 2 h. Thereafter the cells were put into magnetic twisting stimulator and stimulated with magnetic twisting for 2 h. And then the cells were fluorescent stained with FITC-phalloidine. Results The ferromagnetic heads can connected with the cells tightly after 2 h co-incubation. And stress fiher formed after 2 h magnetic twisting stimulation. Conclusion The model of human pulmonary microvascular endothelial cells stretched by magnetic twisting stimulation was an ideal in vitro one to research the response of lung ceils to mechanical stretch.