人类β-防御素2在口腔扁平苔藓病损中表达的研究

Study of the Expressions of Human β-defensin-2 in the Oral Epithelium of Patients with Oral Lichen Planus

目的探讨口腔扁平苔藓(OLP)患者病损中的人类β-防御素2(hBD-2)的表达,以分析hBD-2在OLP发病机制与上皮抗感染免疫中的作用。方法采用免疫组织化学技术(IHC)和反转录聚合酶链式技术(RT-PCR),检测25例OLP病损组织、7例对照组织中hBD-2多肽的表达,并进行分析。结果 hBD-2在所有样本中均出现了阳性胞浆染色,健康口腔黏膜组中hBD-2蛋白为弱阳性染色,与呈强阳性染色的OLP组差异有统计学意义(P<0.05);RT-PCR检测结果显示,所有样本均出现了hBD-2的cDNA电泳条带,在健康口腔黏膜组中呈微弱表达,在OLP组织中表达明显增强,差异有统计学意义(P<0.05)。结论健康口腔黏膜组织也有少量hBD-2表达,提示其作为机体先天性免疫的组成部分,在维持口腔黏膜健康上具有一定作用。在OLP的病理过程中hBD-2可能呈炎性诱导上调表达而参与了OLP的免疫发病过程。

Objective To investigate the expressions of human β-defensin-2（hBD-2）in oral epithelium in patients with oral lichen planus（OLP）,and explain the pathomechanism of hBD-2 in OLP and inflammatory response of epithelium.Methods Oral mucous tissue samples from 25 OLP patients and 7 health controls were included in this study.Immunohistochemistry（IHC）and reverse transcription-polymerase（RT-PCR）were used to evaluate the expression of hBD-2 separately.Results Positive stains of hBD-2 were observed in all the samples by IHC method.The reaction intensity in OLP group was strong,while in health controls it showed weak signal,which showed statistical difference（P0.05）.By RT-PCR,hBD-2 were expressed in cDNA electrophoresis strips both in health controls and patients.The expressions levels of hBD-2 mRNA in OLP patients were significantly higher than those of health controls（P0.05）.Conclusion The small amount of expressions of hBD-2 on normal oral mucosa,which suggests that it play a role in maintaining the health of oral mucosa as a part of innate immunity.The up-regulating expression of hBD-2 under inflammation station in OLP may participate in the immunity course of OLP.