核桃楸树皮提取物抗肿瘤及免疫调节作用的研究

Investigation into Anti-Tumor and Immunomodulatory Effects of Juglans Mandshurica Maxim Extracts(HT)

研究核桃楸树皮提取物（HT）的抗肿瘤及免疫调节作用．建立荷瘤小鼠模型，检测HT抑瘤率及对免疫器官、腹腔巨噬细胞吞噬功能、T淋巴细胞增殖、血清IL一2、TNF-α水平以及机体免疫功能的影响；建立小鼠免疫功能低下模型，检测HT对外周血细胞的影响．实验表明HT具有明显的抑瘤作用，高剂量组抑瘤率达54．6％，可显著提高荷瘤小鼠胸腺及脾脏系数、巨噬细胞吞噬功能、T淋巴细胞增殖、IL-2及TNF-α的表达以及延长游泳及耐缺氧时间，并能升高免疫抑制小鼠的白细胞（p＜0．050～0．001）．HT可有效抑制肿瘤增殖并调节机体的免疫功能．

To study the effects and mechanisms of Juglans mandshurica maxim extracts （HT） on anti-tumor and immunoregulation in mice, the experimental model of tumor-bearing mice was settled. HT performance of tumor inhibition rate, immune organs index, phagocytic function of macrophage, T lymphocyte proliferation, levels of IL-2 and TNF-a in serum and immune functions were tested on the basis of decided model. In addition, the effects of HT on peripheral blood cells were examined in immunosuppressed mice model. Experiment showed that, compared with the model group, HT could inhibit significantly tumor expansion and improve immune organs index. For the subject group with high-dose HT, the tumor inhibition rate was 54.6%, the phagocytic function of macrophage and T lymphocyte proliferation were enhanced, as well as the levels of IL-2 and TNF-a （p〈0. 050～0. 001） were promoted. Moreover, HT could prolong exhausted swimming time and hypoxia tolerance time of the subjects, and increase leukocyte （p〈0. 050～0. 001） of mice. It is obvious that HT has the prominent effects on inhibiting tumor proliferation and regulating immune functions.