广西山绿茶药材HPLC指纹图谱研究

Study on Fingerprints of Ilex hainanensis Merr. by HPLC

目的:建立山绿茶药材HPLC指纹图谱分析方法。方法:采用反相高效液相色谱法,C18柱(5μm,4.6mm×150mm),乙腈-0.1%磷酸溶液梯度洗脱,流速1.0mL/min,检测波长340nm,柱温30℃,使用"计算机辅助相似度评价软件"进行数据处理。结果:建立了山绿茶药材的HPLC指纹图谱,以芦丁为参照峰,确立了山绿茶药材指纹图谱中的12个共有峰。结论:所建立的指纹图谱有较强的针对性,为山绿茶的研究开发以及制定山绿茶的质量标准提供了科学依据。

Objective：To establish the HPLC fingerprints of Ilex hainanensis Merr.Methods：RP-HPLC was used on a C18 column,with a mixture solution of acetonitrile and 0.1%H3PO4 as mobile phase using a gradient elution.The flow rate was 1.0mL/min,the column temperature was 30℃,and the detection wavelength was 340nm.Use the＂Computer Aided Similarity Evaluation＂software to compare the quality of PLT.Results：The HPLC fingerprints of Ilex hainanensis Merr.showed 12 characteristic peaks,the reference peak is Rutin.Conclusion：The fingerprint has pertinency.It can be used for the foundation of quality control and further research of Ilex hainanensis Merr.