摘要
目的:建立山绿茶药材HPLC指纹图谱分析方法。方法:采用反相高效液相色谱法,C18柱(5μm,4.6mm×150mm),乙腈-0.1%磷酸溶液梯度洗脱,流速1.0mL/min,检测波长340nm,柱温30℃,使用"计算机辅助相似度评价软件"进行数据处理。结果:建立了山绿茶药材的HPLC指纹图谱,以芦丁为参照峰,确立了山绿茶药材指纹图谱中的12个共有峰。结论:所建立的指纹图谱有较强的针对性,为山绿茶的研究开发以及制定山绿茶的质量标准提供了科学依据。
Objective:To establish the HPLC fingerprints of Ilex hainanensis Merr.Methods:RP-HPLC was used on a C18 column,with a mixture solution of acetonitrile and 0.1%H3PO4 as mobile phase using a gradient elution.The flow rate was 1.0mL/min,the column temperature was 30℃,and the detection wavelength was 340nm.Use the"Computer Aided Similarity Evaluation"software to compare the quality of PLT.Results:The HPLC fingerprints of Ilex hainanensis Merr.showed 12 characteristic peaks,the reference peak is Rutin.Conclusion:The fingerprint has pertinency.It can be used for the foundation of quality control and further research of Ilex hainanensis Merr.
出处
《辽宁中医药大学学报》
CAS
2011年第6期8-10,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
广西自然科学基金项目(桂科自054297)
关键词
山绿茶
芦丁
HPLC
指纹图谱
Ilex hainanensis Merr.; rutin; HPLC; fingerprint;