黄芩苷与橙皮苷对顺铂所致小鼠肝损伤的保护作用比较

Comparative Effects of Baicalin and Hesperidin on Hepatotoxicity Induced by Cisplatin in Mice

目的:研究黄芩苷与橙皮苷对顺铂所致小鼠肝损伤的保护作用,并比较二者的作用。方法:将ICR小鼠分为4组,即正常组、顺铂模型组、黄芩苷组和橙皮苷组,给药组分别给予黄芩苷(300mg/kg)和橙皮苷(300mg/kg),连续灌胃7天,正常组和模型组给予同体积溶剂,在第3天给药后1h时腹腔注射顺铂(5mg/kg)连续5天,末次注射15h后断头处死小鼠,取肝脏和血液进行检测,通过肝脏的病理组织学检测、谷丙转氨酶(ALT)、谷草转氨酶(AST)测定可反映顺铂引起肝脏损伤,利用生化法测定肝组织丙二醛(MDA)、超氧化物岐化酶(SOD)和谷胱甘肽(GSH)可反映其氧化损伤情况。结果:黄芩苷和橙皮苷可显著抑制顺铂引起小鼠血清ALT和AST水平升高,减少MDA的形成,恢复抗氧化酶SOD的活性,并抑制GSH的耗竭,但黄芩苷的作用比橙皮苷更显著。此外,黄芩苷和橙皮苷明显减轻肝脏水样空泡样变性、炎症浸润和坏死。结论:黄芩苷与橙皮苷均对顺铂所致小鼠肝损伤均具有明显保护作用,尤其是黄芩苷,其保护作用主要与抗氧化作用密切相关。

Objective：The purpose of this study was to investigate the effect of baicalin and hesperidin on hepatotoxicity induced by cisplatin.Methods： Baicalin（300mg/kg）and hesperidin（300mg/kg,were given orally to mice once a day for 7 consecutive days,respectively.From the third day,cisplatin（5mg/kg,intraperitoneally）was injected at 1h after baicalin and hesperidin administration for 5 consecutive days.Liver injuries were evaluated by biochemical and histopathological examinations at 15h after the last dose of cisplatin.Malondialdehyde（MDA）,glutathione（GSH）and superoxide dismutase（SOD）contents in liver tissue were spectrophotometrically measured.Results： baicalin and hesperidin pretreatment significantly inhibited the elevations of serum ALT,and AST levels,alleviated the formation of MDA,restored impaired antioxidant SOD,and suppressed GSH depletion in liver in cisplatin intoxicated mice.Conclusion： The results demonstrated that baicalin and hesperidin had a remarkable protective effect on hepatotoxicity induced by cisplatin,especially baicalin,which was mainly due to its ability to attenuate oxidative stress.