甘薯种质资源遗传稳定性及遗传多样性SSR分析

Genetic Stability and Diversity of Sweetpotato Germplasm with SSR

应用SSR标记检测国家种质徐州甘薯试管苗库中离体保存5年和8年的24份种质资源及其对应的田间圃材料的遗传稳定性,同时对24份甘薯种质的遗传多样性进行分析。20对SSR引物分析表明,24份甘薯材料扩增得到了清晰的DNA条带30条,其中多态性条带21条,多态性百分率为70%,全部品种在2种保存方式下谱带一致,说明2种保存方式的效果相同。应用NTSYS软件对材料进行遗传相似性和UPGMA聚类分析,24份甘薯种质资源遗传相似系数在0.57~0.93之间,平均为0.74。在0.72的相似系数上24份材料可以聚成三大类,表明我国的甘薯品种种质资源遗传多样性还是比较丰富的。该研究为甘薯种质资源长期离体保存及甘薯杂交育种提供了理论依据。

Genetic stability of 24 sweetpotato germplasm maintained in vitro more than 5 years in the National Sweetpotato in vitro Genebank in Xuzhou,was analyzed using Simple Sequence Repeat（SSR）molecular markers.20 pairs SSR primers were used.The results showed that all sweetpotato germplasm stored in vitro had the same amplified products as the controls preserved in field nursery,indicating that the two different methods have the same effect on preserving sweetpotato germplasm stability.Meanwhile,we analyzed the genetic diversity of 24 sweetpotatoe germplasm.The dendrogram generated by UPMGA showed the genetic relationship among 24 accessions.The genetic similarity among 24 accessions varied from 0.57 to 0.93.The total number of bands and polymorphic bands in the 20 primer combinations were 30 and 21 respectively.The 24 accessions were divided into 3 groups by cluster analysis.The results may provide a theoretical basis for sweetpotato germplasms maintained in vitro and the breeding of sweetpotato.