红根甘肃桃根系MYB基因片段的克隆及序列分析

Cloning and Characterization of MYB Genes from Root System of Honggengansutao Peach(Prunus kansuensis L.)

为了明确MYB转录因子在红根甘肃桃抗性分子机制中的作用,通过RT-PCR从红根甘肃桃根系cDNA中克隆了14个MYB基因片段,序列分析表明这些片段与苹果、葡萄、拟南芥、番茄等植物的MYB转录因子高度同源;系统进化分析显示红根甘肃桃的11个PkMYB分别与拟南芥、番茄、杨树、柿等植物中已知功能的MYB转录因子聚在不同的亚类,据此推测它们可能有相似的功能。试验结果为进一步研究MYB基因在红根甘肃桃抗性机制中的作用奠定了基础。

In order to identify the molecular resistance mechanism of Honggengansutao peach（Prunus kansuensis L.）,14 fragments in MYB genes family were isolated from cDNA in root system of Honggengansutao peach by RT-PCR.By comparison,we found that their inferred amino acid sequences have high identity with their counterparts from apple,grape,Arabidopsis,tomato and other plants in GenBank.The result of phylogenetic analysis showed that 11 PkMYBs are clustered with known functions MYBs from Arabidopsis,tomato,poplar and persimmon in different subgroups.According to above results,we predicted that PkMYBs have similar functions with other MYB proteins.These consequences will provide a foundation of coming research about MYB gene function in resistant mechanism of Honggengansutao peach.