利什曼原虫抗原对草原兔尾鼠感染婴儿利什曼原虫的免疫保护作用

PROTECTIVE EFFECTS OF LEISHMANIAL ANTIGENS AGAINST LEISHMANIA INFANTUM INFECTION IN LAGURUS LAGURUS *

目的： 测定利什曼原虫主要表面分子抗原对内脏利什曼病的免疫保护作用。方法： 用重组利什曼原虫表面糖蛋白ｒ Ｇ Ｐ６３ 和脂磷酸聚糖（ Ｌ Ｐ Ｇ） 为抗原， 以短棒状杆菌菌苗（ Ｃ Ｐ） 为佐剂免疫草原兔尾鼠后， 用婴儿利什曼原虫强毒株攻击， 观察免疫保护效果。结果： ｒ Ｇ Ｐ６３ 加 Ｌ Ｐ Ｇ 加 Ｃ Ｐ 抗原组合免疫后用２ ×１０７ 前鞭毛体攻击， 免疫动物的肝印片上 Ｌ Ｄ 数量明显降低， 减虫率为８９８ ％ 。 Ｌ Ｐ Ｇ 加 Ｃ Ｐ 免疫组减虫率为６０６ ％ 。ｒ Ｇ Ｐ６３ 包涵体加 Ｃ Ｐ 免疫组减虫率为４２４ ％ ， 而纯化ｒ Ｇ Ｐ６３ 加 Ｃ Ｐ 免疫组未显示保护作用。以ｒ Ｇ Ｐ６３ 加 Ｌ Ｐ Ｇ 加 Ｃ Ｐ 免疫后用１ ×１０６ ， ５ ×１０６ 和１ ×１０７ 前鞭毛体攻击时， 感染率亦有明显降低。结论： 利什曼原虫主要表面分子抗原 Ｇ Ｐ６３和 Ｌ Ｐ Ｇ 在以 Ｃ Ｐ 为佐剂的条件下， 对草原兔尾鼠婴儿利什曼原虫有明显的免疫保护效果。

AIM: : To determine the protective effect of leishmanial surface antigens against experimental visceral leishmaniasis in Lagurus lagurus caused by Leishmania infantum .METHODS: Recombinant surface protein (rGP63) and lipophosphoglycan (LPG) of Leishmania were used with Corinebacterium parum vaccine as ajuvant to immunize Lagurus lagurus against a challenge with virulent strain of Leishmania infantum . The efficacy of immunoprotection was observed.RESULTS: When challenged with up to 2×10 7 promastigotes, the number of LD on the liver printing sections in the rGP63+LPG+CP immunised animals was significantly decreased ,the parasite reduction rate being 89\^79%. LPG+CP gave a parasite reduction rate of 60\^6% and rGP63/β\|galactosidase fusion protein +CP showed a parasite reduction rate of 42\^45%.Purified rGP63 showed no protection. Immunization with rGP63+LPG+CP followed by challenge inifection with 1×10 6,5×10 6 and 1×10 7 promastigotes also showed significantly reduced infection rates.CONCLUSION: A combination of rGP63+LPG+CP antigens could provide significant immunoprotection against L.infantum challenge in L.lagurus .