尖锐湿疣患者中趋化因子受体CXCR1、CXCR4及其配体的水平

Chemokine receptors CXCR1,CXCR4 and ligands levels in patients with condyloma acuminatum

目的:测定趋化因子受体CXCR1与CXCR4及其配体在尖锐湿疣(CA)患者中的水平。方法:病程大于3个月的CA患者30例,实时定量PCR法检测外周血单个核细胞(PBMC)及皮损中CXCR1与CXCR4mRNA表达水平,流式细胞仪分析外周血白细胞CXCR1与CXCR4的表达情况,ELISA检测血清IL-8与SDF-1α的水平;设30例健康人为正常对照。结果:CX-CR4 mRNA与SDF-1α在CA患者中的水平显著高于对照组(均P<0.01)。结论:CXCR4与SDF-1α可能参与了CA的发病机制。

Objective：To determine the levels of chemokine receptors CXCR1,CXCR4 and IL-8,SDF-1α in codyloma acuminatum（CA） patients.Methods： Real-time quantitative PCR technique was applied to quantitatively analyze CXCR1 and CXCR4 mRNA expression levels in peripheral blood mononuclear cells（PBMC） and lesions,two-colour flow cytometry was used to detect CXCR1 expression cells in blood leucocytes,serum levels of IL-8 and SDF-1α were measured by double antibody sandwich enzyme-linked immunosorbent assay（ELISA） in 30 cases of diagnosed CA patients（ 3 months of the course of disease） and 30 healthy controls.Results：The CXCR4 and SDF-1α levels in CA patients were significantly higher than that of the normal controls（P0.01）.Conclusion：The increased CXCR4 and SDF-1α may be involved in the pathogenesis of CA.