子宫内膜异位症异位内膜细胞原代培养方法的改良

Improvement of primary culture methods for human ectopic endometrial cells from patients with endometriosis

目的:建立一种简易的人异位子宫内膜细胞体外原代培养方法。方法:通过胰蛋白酶消化、贴壁纯化等技术,分离及培养10例子宫内膜异位症患者的异位子宫内膜细胞。在光学显微镜下观察细胞形态,以免疫细胞化学法鉴定细胞类型。结果:8份标本获得成功,培养的异位内膜细胞均有腺上皮细胞和间质细胞两种形态细胞。膜上皮细胞和基质细胞分别经角蛋白单抗和波形蛋白单抗免疫组化染色为阳性,腺上皮细胞平均生长时间为5周,间质细胞平均生长时间为14周。结论:成功建立了子宫内膜异位症异位内膜细胞体外原代培养方法,该改良培养法经济、简单、高效。

Objective：To explore a convenient method for primary culture of ectopic endometrial cells from patients with endometriosis.Methods： The ectopic endometrial cells from ten patients with endometriosis were isolated and purified by trypsin digestion and pasted wall purification.The cellular morphology was observed by optical microscope.Endometrial epithelial cell（EEC） and endometrial stromal cell（ESC） were identified by immunocytochemistry.Results：The cell culture was successfully established in 8 of 10 cases of ectopic endometrium.Both EEC and ESC were existed in the culture and stained positively by cytokeratin and vimentin monoclonal antibody,respectively.The life span of EEC and ESC can last 5 weeks and 14 weeks respectively.Conclusion：The ectopic endometrial cells from patients with endometriosis were successfully cultured in vitro.The improved primary culture method for ectopic endometrial cells is convenient and of high performance.