阿托伐他汀对心脏成纤维细胞MMP-9/TIMP-1表达的影响

Effects of atorvastatin on the expression of MMP-9/TIMP-1 in cardiac fibroblasts

目的:观察阿托伐他汀(atorvastatin)对心脏成纤维细胞基质金属蛋白酶-9/组织型基质金属蛋白酶抑制因子-1(MMP-9/TIMP-1)表达的影响。方法:体外培养乳鼠心脏成纤维细胞(CF),做细胞形态观察和心脏成纤维细胞标志性波形蛋白免疫荧光组化检测,明胶酶谱法测定MMP-9的活性,RT-PCR检测MMP-9和TIMP-1mRNA的表达。结果:AngⅡ增加MMP-9的活性及mRNA的表达(P<0.05),同时TIMP-1的mRNA表达减少(P<0.05),阿托伐他汀在某种程度上可逆转上述变化(P<0.05)。结论:Aorvastatin通过对MMP-9/TIMP-1表达的影响作为预防和治疗房颤的重要机制之一。

Objective：To study the effects of atorvastatin on the expression of MMP-9/TIMP-1 in cardiac fibroblasts（CF）.Methods： Cardiac fibroblasts of neonatal rats were cultured in vitro,cell morphology was observed.As one of the characters of CFS,protein vimentin was detected with immunofluorescence histochemistry.The activity of MMP-9 was measured by gelatinase zymograph,and mRNAs of MMP-9 and TIMP-1 were detected by RT-PCR.Results：The activity and the mRNA expression of MMP-9 were increased by AngⅡ（P0.05）,while the expression of TIMP-1 was decreased.Atorvastatin can reverse the above changes to some extent.Conclusion：Through modulating the expression of MMP-9/TIMP-1,atorvastatin may play an important role in the prevention and treatment of atrial fibrillation.