补肾益精中药对老龄小鼠活性基因及核糖核酸酶活性的影响

The Ageing delaying effects of Chinese herb medicines which possess the properties of Bu Shen Yi Jing on nucleic active genes and RNAase activity of old mice

目的 研究补肾益精药物对老龄小鼠肝细胞核活性基因、血清核糖核酸酶及血 Ｓ Ｏ Ｄ 活性的影响，初步探讨补肾益精药物延缓衰老的分子机理。方法 用紫外分光光度法和电泳法检测活性基因，用紫外发光光度法检测 Ｒ Ｎ Ａ ａｓｅ 活性，用化学发光法检测 Ｓ Ｏ Ｄ活性。结果 青龄小鼠肝细胞核对活性基因探针 Ｄ Ｎ Ａ ａｓｅⅠ的消化敏感性、血 Ｓ Ｏ Ｄ 活性显著高于老龄对照组，血清 Ｒ Ｎ Ａ ａｓｅ 活性较老龄对照组显著降低；服用了补肾益精药物的老龄小鼠其肝细胞核对 Ｄ Ｎ Ａ ａｓｅⅠ消化敏感性和血 Ｓ Ｏ Ｄ 活性较老龄对照组均显著升高，血清 Ｒ Ｎ Ａ ａｓｅ 活性较老龄小鼠显著降低。结论 补肾益精药物益肾宝及左归饮使老龄小鼠肝细胞核中的活性基因数及血 Ｓ Ｏ Ｄ 活性升高，血清 Ｒ Ｎ Ａ ａｓｅ 活性降低，这可能是补肾益精药物延缓衰老的分子机理之一。

Effect of administration pathway and time vaviation of nimodipine on the neuromonoamine transmitter changes in hippocampal gyrus during cerebral ischemia injury$$$$ Zhu Xiaobo, Liu Baohuan, Wang Xiaoming et al The First Clinical Hospital of Norman Bethune University of Medical Science, Changchun 130021 In the experiment, the level of serum neuromonoamine transmitter in hippocampal gyrus in cerebral ischemiareperfusion using nimodipine by local therapy and general therapy in different time were measured. The results showed that treatment effect of early using nimodipine in reperfusion was better than that of the late, treatment effect of using nimodipine by local therapy was better than that of general therapy. Key words Cerebral ischemia reperfusion Nimodipine Neurous trans mitter