药物诱发大鼠痴呆模型的初步研究

The experiment of drug induced dementia model of the rats

目的 建立一种模拟痴呆动物模型的制备方法。方法 按二水平三因素 Ｌ１６ （２１５ ）正交试验设计分组，应用 Ａ 因素（东莨菪碱）、 Ｂ 因素（氯胺酮）、 Ｃ 因素（ Ｄ半乳糖）皮下注射制备动物模型。利用 Ｍ Ｇ２ 迷宫检测大鼠学习记忆功能。通过组织病理学观察大鼠海马 Ｃ Ａ １ 区锥体细胞的数量和形态及神经元纤维缠结和老年斑等。结果 氯胺酮和 Ｄ 半乳糖分别可致大鼠记忆功能明显下降（ Ｐ ＜ ００５）和海马 Ｃ Ａ １ 区锥体细胞减少，并可见嗜神经细胞现象和胶质细胞小结。结论 大鼠注射氯胺酮或 Ｄ半乳糖可作为模拟痴呆模型的方法之一。

Objective Try to prepare an animal model of senile dementia with learning & memory disorders.Methods The model was made by the injection of medicine according to 2 levels and 3 elements of L 16 (2 15 ) orthogonal experimental design. The elements A(scopolamine), B(phencyclidine), C(D galactose) were used as the injected drugs to the rats. The ability of learning & memory was tested by using the MG 2 type maze. The amounts and morphon of pyramidale, the neurofibrillary tangle(NFT) and senior plaque(SP) were observed.Results B, C can cause the hypomnesia of the Wistar rats (P<0 05) and result in decreasing amounts of pyramidale in CA1 sector of hippocampus. The neurotropic and glial nodule were observed.Conclusions The injection of Phencyclidine of D galactose may be one of the methods of preparing dementia model of rats with learning & memory disorders.