腺苷A2A受体激活缺血后处理保护皮瓣作用研究

Study on the role of the ischemia postconditioning with adenosine A2A receptor agonist

目的:缺血后处理需要反复夹闭血管蒂,可能对血管造成机械性损伤。药物缺血后处理成为缺血后处理的发展方向。腺苷A2A是调控皮瓣炎症的关键靶点,进行相关研究进一步探究腺苷A2A激活缺血后处理对皮瓣有否保护作用。方法:健康成年新西兰大白兔,分为3组。A组为假手术组;B组为缺血再灌注损伤组;C组,再灌注前5min注射腺苷A2A激活剂。分别进行皮瓣存活率检测和ELISA检测TNF以及IL-6。结果:腺苷A2A激活剂组皮瓣存活率高,炎症因子检测活性低于缺血再灌注损伤组。结论:腺苷A2A激活剂缺血后处理可以抑制炎症因子,具有保护皮瓣作用。运用腺苷A2A受体激活缺血后处理可能成为保护皮瓣的新措施。

Objective Ischemia postconditioning has been used performed on the vascular pedicle with repeated clamping and loosing which may compromise the vascular.The drug postconditioning is recorded as a future development,widely among heart and kidney and so on.Adenosine A2A is a key target to control the inflammation of the skin flap.So further research is performed to explore whether adenosine A2A postconditioning can protect the skin flap.Methods The healthy new Zealand rabbits are divided into 3 groups.A group is defined as sham group;B group is ischemia reperfusion group;C group,adenosine A2A is injected 5 minutes before the reperfusion..Then the examinations are held about the survival skin flap、TNF and IL-6.Results B group has a more survival percentage area of skin flap.The inflammation factor is lower than the ischemia-reperfusion group.Conclusion Adenosine A2A postconditioning can inhibit the inflammation factor and protect the skin flap.It can be a new thearptic measure to protect the skin flap with adenosine A2A postconditioning.