杜氏利什曼原虫无鞭毛体蛋白基因原核表达重组质粒的构建

Construction of Prokaryotic Expression Recombinant Plasmid of Amastin Gene of Leishmania donovani

目的:构建杜氏利什曼原虫无鞭毛体蛋白(amastin)编码基因的原核表达重组质粒pET-amastin。方法:提取杜氏利什曼原虫基因组DNA进行PCR扩增,将扩增出的无鞭毛体蛋白基因片段导入质粒载体pET-32a(+),构建原核表达重组质粒pET-amastin。结果:扩增出大小约550 bp的无鞭毛体蛋白基因,重组质粒pET-amastin经鉴定正确。结论:成功构建杜氏利什曼原虫无鞭毛体蛋白基因原核表达重组质粒pET-amastin。

Objective： To construct prokaryotic expression recombinant plasmid of amastin gene of Leishmania donovani.Methods： Amastin gene in nuclear DNA of L.donovani was isolated and amplified which was then cloned into prokaryotic expression vector pET-32a（＋） to construct recombinant plasmid pET-amastin.Results： The amastin gene of 550bp was acquired by PCR.The recombinant plasmid pET-amastin was identified to be correct.Conclusion： The prokaryotic expression recombinant plasmid of amastin gene of L.donovani has been constructed successfully.