海洛因依赖大鼠海马CA3区PS幅值的变化

A Study on Long-term Potentiation Changes in Hippocampus CA3 Area of Heroin Dependent Rats

目的:应用细胞外电生理记录方法观察海洛因依赖大鼠海马CA3区的长时程增强(LTP)变化。方法:将40只SD大鼠随机分成海洛因依赖组、海洛因+MK801组、海洛因戒断组及空白对照组,在体记录由刺激海马外侧穿通纤维(lateral perforant path,LPP)所诱发的CA3区细胞群体峰电位幅值(PS幅值)。结果:高频刺激后第1、5、10、20、30、40、50、60 min时点的PS幅值变化率在戒断组明显低于对照组?海洛因依赖组和海洛因+MK801组,差异有显著性;海洛因依赖组、对照组与海洛因+MK801组的差异无显著性。结论:海洛因戒断大鼠海马CA3区LTP效应受损。

Objective： To observe the long-term potentiation（LTP） changes in hippocampus CA3 area of heroin dependent rats with the method of extracellular electrophysiological recording.Methods： Forty SD rats were randomly divided into four groups： heroin dependent group（group HD）,heroin＋MK-801 group（group HM）,heroin abstinence group（group HA） and control group（group C）.Population spikes of cells in CA3 region of each group were recorded in vivo.LTP effect induced by high-frequency stimulations was observed.Results： After tetanic stimulation,the change rates of population spike in group HA were significantly lower than those in groups HM,HD and C at time points of 1,5,10,20,30,40,50,and 60 minutes,and no significant difference was found among the latter 3 groups.Conclusions： The LTP effect is impaired in heroin abstinence rats.