电磁辐射对N9小胶质细胞STAT3核转位以及磷酸化水平的影响

Effect of electromagnetic irradiation induced STAT3 DNA binding and phosphorylation in N9 microglial cells

目的研究STAT3信号分子是否参与电磁辐射诱导的小胶质细胞活化。方法Western-blot检测小胶质细胞STAT3蛋白质表达及磷酸化表达变化，凝胶迁移率实验（Electrophoretic Mobility Shift Assay，EMSA）检测小胶质细胞STAT3的DNA结合活性的变化。结果电磁辐射后小胶质细胞STAT3磷酸化增强，STAT3核转位与DNA结合活性在辐照后6-24h增加，并以12h最为明显。结论STAT3信号分子参与了电磁辐射诱导的小胶质细胞活化过程。

Objective To investigate the possible participation of STAT3 signaling molecule and activation of microglia after electromagnetic irradiation. Methods Phosphorylation of STAT3 was tested using western blotting. STAT3 DNA binding activity was detected by EMSA. Results After electromagnetic irradiation, the phosphorylation of STAT3 was detected rapidly post microwave exposure. A significant increase of STAT3 DNA-binding ability was noted from 6b to 24h after exposure, especially at 12h. Conclusion The STAT3 signaling molecule may participate in the activation of microglia induced by electromagnetic irradiation.